The chemolithoautotrophic strain Beggiatoa sp. 35Flor shows an unusual migration behavior when cultivated in a gradient medium under high sulfide fluxes. As common for Beggiatoa spp., the filaments form a mat at the oxygen-sulfide interface. However, upon prolonged incubation, a subpopulation migrates actively downward into the anoxic and sulfidic section of the medium, where the filaments beco...

The mixotrophic growth with methanol plus thiosulfate was examined in nutrient-limited mixotrophic condition for Methylobacterium goesingense CBMB5 and Methylobacterium fujisawaense CBMB37. Thiosulfate oxidation increased the growth and protein yield in mixotrophic medium that contained 150 mM methanol and 20 mM sodium thiosulfate, at 144 h. Respirometric study revealed that thiosulfate was the...

Ergothioneine was synthesized and accumulated in growing cultures of Mycobacterium smegmatis when the medium was adequately supplied with sulfur. In a low sulfur medium, the accumulation was sharply limited although growth of the organism was apparently normal. Synthesis of hercynine, the precursor of ergothioneine, was unaffected by low sulfur levels and was markedly increased by addition of l...

The formation of periplasmic sulfur globules is an intermediate step during the oxidation of reduced sulfur compounds in various sulfur-oxidizing microorganisms. The mechanism of how this sulfur is activated and crosses the cytoplasmic membrane for further oxidation to sulfite by the dissimilatory reductase DsrAB is incompletely understood, but it has been well documented that the pathway invol...

Mutation of a serine that forms a hydrogen bond to the iron-sulfur cluster of the Rieske iron-sulfur protein to a cysteine results in a respiratory-deficient yeast strain due to formation of iron-sulfur protein lacking the iron-sulfur cluster. The Rieske apoprotein lacking the iron-sulfur cluster is inserted into both monomers of the dimeric cytochrome bc(1) complex and processed to mature size...

Naphtho[2,1-b]thiophene (NTH) is an asymmetric structural isomer of dibenzothiophene (DBT), and in addition to DBT derivatives, NTH derivatives can also be detected in diesel oil following hydrodesulfurization treatment. Rhodococcus sp. strain WU-K2R was newly isolated from soil for its ability to grow in a medium with NTH as the sole source of sulfur, and growing cells of WU-K2R degraded 0.27 ...

The most striking result of a study of the process of chemosynthesis (synthesis of all materials from CO2 using chemical energy obtained by oxidation) in the autotrophic bacterium, Thiobacillus thiooxidans, was the ability of the cell to store up the energy derived from sulfur oxidation in an available form which could be used for COs fixation under conditions in which sulfur oxidation was impo...

Streptomyces sioyaensis, which produces the antibiotic siomycin, oxidizes elemental sulfur when added to the culture medium and accumulates thiosulfate in the fermented broth. The accumulated thiosulfate was isolated as the ammonium salt and was identified by melting point, IR spectrum, and paper chromatography. A variety of other streptomycetes also oxidized elemental sulfur and accumulated th...

I This contribution is one of a series of papers by the Multiple Fellowship on Mine Acid Control sustained at Mellon Institute by the Sanitary Water Board, Department of Health, Commonwealth of Pennsylvania. catalyst. Indeed, Carpeniter and Heirndon (1933) suggested that bacterial activity by some sulfur oxidizing organism might explain, in part, the high acidities usually encountered in bitumi...

I This contribution is one of a series of papers by the Multiple Fellowship on Mine Acid Control sustained at Mellon Institute by the Sanitary Water Board, Department of Health, Commonwealth of Pennsylvania. catalyst. Indeed, Carpeniter and Heirndon (1933) suggested that bacterial activity by some sulfur oxidizing organism might explain, in part, the high acidities usually encountered in bitumi...