Association of alteration in DNA methylation pattern in triggering 12O-tetradecanoylphorbol-13-acetate (TPA (-induced transcription of ornithine decarboxylase (ODC) gene in T24 cells was determined. In accord with our previous findings (Archiv. Biochem. Biophys., 262: 326-336, 1988), TPA treatment of I 24 cells, cultured in serum-free medium, resulted in a dramatic (~15-fold) increase in ODC ac...

Apoptosis is a process that leads to programmed cell death and also a therapeutic target of cancer. In this study, potential apoptotic effects of shikonin on human bladder cancer cells (T24) in vitro were evaluated. Apoptosis induction, cell viability and morphological changes were investigated and caspase-3 and -9 activity was determined by flow cytometric assay and reverse transcription-polym...

We designed a model system to study the role of von Willebrand factor (vWF) in the sorting of P-selectin and the biogenesis of Weibel-Palade body (WPB)-like organelles. For that purpose, a human epithelial cell line (T24) that synthesizes P-selectin mRNA, but which is devoid of vWF mRNA synthesis and storage organelles, was transfected with full-length vWF cDNA or a deletion mutant thereof. Sta...

The epidermal growth factor receptor (EGFR) is associated with aggressive phenotypes and is an independent predictor of stage progression and mortality in bladder cancer. Gefitinib ('Iressa,' ZD1839) is an orally active EGFR-tyrosine kinase inhibitor. The objective of this study was to evaluate the in vitro and in vivo effects of gefitinib in the EGFR-expressing human bladder cancer cell lines ...

because of difference in the toxicokinetic and pharmacokinetic characteristics of drugs and with respect to the high rate of benzodiazepine abuse in suicidal attempts, the trend of possible anterograde amnesia after recovery from benzodiazepine overdose were evaluated in iranian patients. this was a prospective descriptive/analytic clinical trial, which was conducted, in noor general teaching h...

BACKGROUND Late-onset sepsis in very low birth weight (VLBW) infants is a diagnostic challenge. We aimed to evaluate the diagnostic utility of the C-Reactive protein (CRP) and the complete blood count (CBC) for late-onset sepsis in VLBW infants. METHODS In a 5-year retrospective cohort of 416 VLBW infants born at less than 1500 g, there were 590 separate late-onset sepsis evaluations. CRP and...

BACKGROUND Although the satellite cell (SC) is a key regulator of muscle growth during development and muscle adaptation following exercise, the regulation of human muscle SC function remains largely unexplored. STAT3 signalling mediated via interleukin-6 (IL-6) has recently come to the forefront as a potential regulator of SC proliferation. The early response of the SC population in human musc...

Resistance to conventional anticancer chemotherapeutic agents remains one of the major problems in the treatment of bladder cancer. Hence, new therapeutic modalities are necessary to treat the drug-resistant cancers. Apo-2 ligand (Apo-2L) is member of the tumor necrosis factor ligand family, and it induces apoptosis in cancer cells. Several cytotoxic anticancer drugs, including Adriamycin (ADR)...

conclusions the proliferation and invasion of human bladder cancer cells can be inhibited by rnai-targeting skp2. as a result, skp2 may be a potential target for gene therapy in cases of human bladder cancer. materials and methods the expression of the skp2 gene was knocked down by rna interference (rnai) in t24 cells. the transcription level of skp2 was detected by reverse transcription-quanti...

Radiation therapy (RT) delivers tumour kill, directly and often via bystander mechanisms. Bladder toxicity is a dose limiting constraint in pelvic RT, manifested as radiation cystitis and urinary symptoms. We aimed to investigate the impact of radiation-induced bystander signaling on normal/cancer urothelial cells. Human urothelial cancer cells T24, HT1376 and normal urothelial cells HUC, SV-HU...