Trehalose is a disaccharide essential for the survival and virulence of pathogenic fungi. The biosynthesis of trehalose requires trehalose-6-phosphate synthase, Tps1, and trehalose-6-phosphate phosphatase, Tps2. Here, we report the structures of the N-terminal domain of Tps2 (Tps2NTD) from Candida albicans, a transition-state complex of the Tps2 C-terminal trehalose-6-phosphate phosphatase doma...

Interaction between the embryo and endosperm affects seed development, an essential process in yield formation crops such as rice. Signals that mediate communication are largely unknown. We used notched-belly (NB) mutant with impaired to investigate effect of on developmental staging signaling pathways regulate development. Hierachical clustering mRNA datasets from samples collected during deve...

This work studied the precipitation characteristics of AlSi10Mg0.3Mn alloy produced by high-pressure vacuum die-casting in T5 and T6 conditions using transmission electron microscopy, differential scanning calorimetry both electrical conductivity microhardness measurements. Two different tempers were used, involving partial solutionizing at 460 °C for 1 h full 500 h, designated as T6P T6F, resp...

The Sugar Sensor, Trehalose-6-Phosphate Synthase (Tps1), Regulates Primary and Secondary Metabolism during Infection by the Rice Blast Fungus: Will Magnaporthe oryzae's " Sweet Tooth " become Its " Achilles' Heel " ?" (2011). Fungal Molecular Plant-Microbe Interactions. Paper 6. disparate cellular activities? To address this question, this review discusses our current understanding of tre-halos...

Metabolism of the alpha-1,1 glucose disaccharide, trehalose, is indispensable in plants. In the Murashigeand Skoog (MS) medium, trehalose inhibits plant growth and allocation of carbon to roots. A suppressorof trehalose-6-phosphate (T6P) mediated growth arrest, GR-RBP2, is characterized in more detail.Phylogenetic analysis revealed that GR-RBP2 is a protein of likely prokaryot...

BACKGROUND Natural and industrial environments are dynamic with respect to substrate availability and other conditions like temperature and pH. Especially, metabolism is strongly affected by changes in the extracellular space. Here we study the dynamic flux of central carbon metabolism and storage carbohydrate metabolism under dynamic feast/famine conditions in Saccharomyces cerevisiae. RESUL...

Trehalose fulfils a wide variety of functions in cells, acting as a stress protectant, storage carbohydrate and compatible solute. Recent evidence, however, indicates that trehalose metabolism may exert important regulatory roles in the development of multicellular eukaryotes. Here, we show that in the plant pathogenic fungus Magnaporthe grisea trehalose-6-phosphate (T6P) synthase (Tps1) is res...

Trehalose metabolism and its intermediate trehalose-6-phosphate (T6P) are implicated in sensing and signalling sucrose availability. Four class I TREHALOSE-6-PHOSPHATE SYNTHASE (TPS1) genes were identified in kiwifruit, three of which have both the TPS and trehalose-6-phosphate phosphatase (TPP) domain, while the fourth gene gives rise to a truncated transcript. The transcript with highest sequ...

A large body of evidence shows that sugars function both as nutrients and signals to regulate fruit and seed set under normal and stress conditions including heat and drought. Inadequate sucrose import to, and its degradation within, reproductive organs cause fruit and seed abortion under heat and drought. As nutrients, sucrose-derived hexoses provide carbon skeletons and energy for growth and ...