Sugarcane is a major crop for sugar production around the world. The complexity of sugarcane genome creates challenges use both conventional and molecular breeding methods to improve at genetic level. DNA sequencing an important tool assess how genus Saccharum genera complex are interrelated. Here, we identify kinship Nepal2013-6 (Saccharum spontaneum, x = 10) using tetra-primer amplification r...

background congenital factor xiii (fxiii) deficiency is a rare severs autosomal recessive bleeding disorder. objectives the aim of the study was to determine the c559t > c fxiiia genotype frequency in patients with fxiii hemophilia who lived in sistan and balouchestan province in southeast of iran. patients and methods we determined the genotype of 180 patients with factor xiii hemophilia by te...

Background: The transversion of G to T (G894T) in human endothelial nitric oxide synthase (eNOS) gene has profound effects such as male infertility, recurrent miscarriage, multiple sclerosis and cardiovascular diseases.Objectives: Development of a new Multiplex Tetra-Primer Amplifi cation Refractory Mutation System - Polymerase Chain Reaction (T-ARMS-PCR) for detection of...

Thiopurines are clinically useful in the management of diverse immunological and malignant conditions. Nevertheless, these purine analogues can cause lethal myelosuppression, which may be prevented by prospective testing for variants in the thiopurine S-methyltransferase (TPMT) and, in East Asians, Nudix hydrolase 15 (NUDT15) genes. Two single-tube, tetra-primer amplification refractory mutatio...

Introduction: Estrogen, a crucial hormone during pregnancy, acts through two types of receptors. The estrogen receptor alpha and beta (ESR-α and ESR-β) are more abundant and exists in all human reproductive systems. Association of ESR-α and ESR-β genes polymorphisms has been reported in some reproductive problems such as spontaneous abortion, endometriosis-related infertility, and in vitro fert...

Background Psoriasis is a complex autoimmune disease caused by the interaction of genetic and environmental factors. PTPN22 gene polymorphism has been reported to affect psoriasis susceptibility; however, no data are available for Middle Eastern populations. Objective The aim of this study was to investigate the association of PTPN22 (1858C/T) R620W polymorphism with psoriasis in a Saudi coho...

INTRODUCTION The T to C transition at nucleotide 1565 of the human glycoprotein IIIa (ITGB3) gene represents a genetic polymorphism (PlA1/A2) that can influence both platelet activation and aggregation and that has been associated with many types of disease. Here, we present a newly designed multiplex tetra-primer amplification refractory mutation system - polymerase chain reaction (T-ARMS-PCR)...