In this study, we evaluated the effects of the thawing methodology on sperm function after cryopreservation in pellets. We compared the use of two thawing procedures: method (1) maintaining pellet for 10 min in air at room temperature, then another 10-min period in air at 37°C followed by dilution in a thawing medium; and method (2) immersing the pellets directly in thawing medium at 37°C for 2...

During cold acclimation, winter rye (Secale cereale) plants accumulate pathogenesis-related proteins that are also antifreeze proteins (AFPs) because they adsorb onto ice and inhibit its growth. Although they promote winter survival in planta, these dual-function AFPs proteins lose activity when stored at subzero temperatures in vitro, so we examined their stability in solutions containing CaCl...

Permafrost thawing is likely to change the flow pathways taken by water as it moves through arctic and subarctic landscapes. The location and distribution of these pathways directly influence the carbon and other biogeochemical cycling in northern latitude catchments. While permafrost thawing due to climate change has been observed in the arctic and sub-arctic, direct observations of permafrost...

In this paper, rapid freezing and thawing test of air-entrained fly–ash concrete was carried out. Gas adsorption of concrete pores, mass loss, dynamic elastic modulus, and chloride diffusion coefficient of concrete after different freezing and thawing cycles was tested; scanning electron image was used to study the microscopic pore structure of the specimens. Results show that after certain fre...

In this study, the physicochemical and sensory quality characteristics due to the influence of various thawing methods on electro-magnetic and air blast frozen pork were examined. The packaged pork samples, which were frozen by air blast freezing at -45℃ or electro-magnetic freezing at -55℃, were thawed using 4 different methods: refrigeration (4±1℃), room temperature (RT, 25℃), cold water (15℃...

This study explored the effects of water or brine (2% NaCl, w/v) immersion thawing combined with ultrasound treatment (40 kHz, 150 W) on the quality characteristics of pork. Ultrasound treatment of pork was conducted in two cold media (at 4℃), water and 2% (w/v) brine, respectively. Because the ultrasound treatment caused temperature increase in the media from 4℃ to 16℃, the qualities of pork t...

Poly(viny1 alcohol) (PVA) hydrogels were prepared by treating 15 or 20% aqueous PVA solutions to repeated cycles of freezing for 6 or 12 h at 20°C and thawing for 2 h at 25°C. The resulting gels did not contain any leachable toxic crosslinking agents or other residues. Their adhesive characteristics were examined using tensile experiments in contact with bovine submaxillary mucin. As the number...

reactive oxygen species (ros) generation, induced by the cryopreservation process, can be responsible for mammalian sperm damage. curcumin is known as an effective antioxidant against oxidative stress. the aim of this study was to evaluate the effects of curcumin on sperm count, motility and viability, semen total antioxidant capacity and dna integrity of rat spermatozoa during semen freeze-tha...

Changes in sperm structure and function occur during the processing of semen. The present study was designed to investigate the effect on buck sperm during different stages of semen preparation including dilution, cooling, equilibration and freeze-thawing. Semen ejaculates from three mature bucks (replicates = 5) were diluted with tris-citric acid egg yolk glycerol extender at 37 ºC, cooled to ...