The removal of the 1-carbon of threonine can occur via threonine dehydrogenase or threonine aldolase, this carbon ending up in glycine to be liberated by the mitochondrial glycine cleavage system and producing CO(2). Alternatively, in the threonine dehydratase pathway, the 1-carbon ends up in alpha-ketobutyrate, which is oxidized in the mitochondria to CO(2). Rat hepatocytes, incubated in Krebs...

Weanling crossbred pigs (avg initial wt 6.4 kg) were fed diets containing six levels of threonine to determine the threonine requirement of young pigs fed diets somewhat similar to those used in commercial swine production. The diets (16% crude protein) were composed primarily of grain sorghum, oat groats and soybean meal supplemented with minerals, vitamins, lysine, tryptophan, methionine and ...

Threonine dehydratase, threonine aldolase and threonine dehydrogenase activities were assayed in livers of rats that had been normally-fed, starved for 72 h, fed a high-protein diet or normally-fed and injected with glucagon or cortisone. A modified continuous spectrophotometric assay for threonine aldolase overcame interference resulting from threonine dehydratase activity and revealed that th...

In pigs the extensive threonine utilization by the splanchnic tissues explains the relative inefficiency of dietary threonine conversion for body protein accretion. Two experiments were conducted to estimate the contribution of the portal drained viscera (PDV) and the liver to threonine metabolism and especially catabolism in growing pigs. In the first experiment, four pigs were surgically prep...

Podosphaera xanthii causes powdery mildew of cucumber, and is associated with significant yield quality losses. Development resistant or tolerant varieties the most effective eco-friendly strategy for management. An important host resistance mechanism based on recognition conserved genes, resulting in durable resistance. To determine mechanisms total RNAs were isolated from cultivar Meltem, lin...