Background: Lipase enzymes are of great importance in various industries. Currently, extensive efforts have been focused on exploring new lipase producer microorganism as well as genetic and protein engineering of available lipases to improve their functional features. Methods: For screening lipase-producing lactobacilli, isolated strains were inoculated onto tributyrin agar plates. Molecular ...

Cloning and sequencing of a lipase gene from an indigenous Bacillus pumilus, strain F3, revealed an open-reading frame of 648 nucleotides predicted to encode a protein of 215 residues. Sequence analysis showed that F3 lipase contained a signal peptide composed of 34 amino acids with an H domain of 18 residues. A tat-like motif was found in the signal peptide similar to some other Bacillus pumil...

In this study, solid-state fermentation of two types of agricultural residues/products for lipase production in a tray-bioreactor was investigated. Rhizopus oryzae was used as a potential fungus strain and two types of agricultural residues including soybean meal and sugarcane bagasse were utilized as substrate. Fermentation was carried out in two different operational conditions: one with cont...

Background and Objectives Alkaline pH of the soil facilitates the conversion of phosphate present in phosphate fertilizer applied in the field to insoluble phosphate which is not available to plants. Problem of soluble phosphate deficiency arises, primarily due to needless use of phosphate fertilizer. We sought to biofertilizer with the thermo-tolerant phosphate solubilizing actinomycetes conso...

background: lipase is an enzyme with immense application potential. ester synthesis by lipase catalysis in organic media is an area of key industrial relevance. enzymatic preparations with traits that cater to the needs of this function are hence being intensely researched. objective: the objectives of the study were to immobilize the lipase from bacillus sp. ps35 by cross-linking and adsorptio...