Acetaminophen/paracetamol-induced liver failure--which is induced by the binding of reactive metabolites to mitochondrial proteins and their disruption--is exacerbated by fasting. As fasting promotes SIRT3-mediated mitochondrial-protein deacetylation and acetaminophen metabolites bind to lysine residues, we investigated whether deacetylation predisposes mice to toxic metabolite-mediated disrupt...

Citrus are sensitive to boron (B)-toxicity. In China, B-toxicity occurs in some citrus orchards. So far, limited data are available on B-toxicity-responsive proteins in higher plants. Thirteen-week-old seedlings of "Sour pummelo" (Citrus grandis) and "Xuegan" (Citrus sinensis) was fertilized every other day until dripping with nutrient solution containing 10 μM (control) or 400 μM (B-toxicity) ...

Cancer and neurodegeneration include a group of diseases that are mechanistically distinct but may share common therapeutic targets. Autophagy is a common quality control mechanism shared by mitotic and post-mitotic cells and it can be exploited to accelerate clearance of unwanted oncogenes and reduce accumulation of toxic proteins in cancer and neurodegeneration, respectively. Tyrosine kinase ...

Single-step fusion-based affinity purification of proteins with pH-controllable linkers was carried out in a fluidic device. The linkers were previously derived from self-splicing protein elements called inteins. Two different linkers were generated to solve two distinct separation problems: one for rapid single-step affinity purification of a wide range of proteins, and the other specifically ...

: The venoms of Tunisian wildlife snakes are complex mixtures containing proteins/ peptides and non-protein molecules. Proteins the most abundant compounds responsible for biological effects venoms. Snake proteins have enzymatic or nonenzymatic activities, which grouped into different families, including C-type lectin proteins, disintegrins (long, medium short disintegrins), Kunitz-type serine ...

How are toxic proteins cleared by microglia? Since microglia are the macrophages of the CNS, the promotion of an increase in their ability to phagocytose highly toxic proteins is a promising new therapeutic approach to prevent many diseases. Toxic proteins are produced in a variety of brain diseases, such as Alzheimer’s disease (b-amyloid), amyotrophic lateral sclerosis (superoxide dismutase 1)...

Cys2His2 zinc finger proteins are important for living organisms, as they—among other functions—specifically recognise DNA when Zn(II) is coordinated to the proteins, stabilising their ??? secondary structure. Therefore, competition with metal ions may alter original function. Toxic such Cd(II) or Hg(II) might be especially dangerous because of similar chemical properties Zn(II). Most studies c...