Protein synthesis is the result of a series of coupled events, many of which can be measured. In this report, the determination of the synthesis of peptides of chain length three and greater was used as a measure of translocation, the movement of the ribosome along the polyribonucleotide template. Translocation was observed both in the presence and absence of one added soluble transfer factor w...

PURPOSE To investigate the roles of activation of macrophages isolated from C3H/HeN and C3H/HeJ mice and stimulated by lipopolysaccharide (LPS), and toll-like receptor 4-mediated signal transduction in the development of acute anterior uveitis. METHODS Establish animal models with acute anterior uveitis by intraperitoneal injection of vibrio cholera endotoxin into C3H/HeN mice (wild type) and...

Botulinum neurotoxins and tetanus are the strongest known toxins that cause neuroparalytic syndromes in botulism tetanus. This review aimed to systematize scientific data on structures mechanism of actions botulinum neurotoxins. proteins containing functional domains responsible for receptor binding, transmembrane translocation, proteolytic cleavage required exocytosis synaptic vesicles release...

Elongation factor (EF) G promotes tRNA translocation on the ribosome. We present three-dimensional reconstructions, obtained by cryo-electron microscopy, of EF-G-ribosome complexes before and after translocation. In the pretranslocation state, domain 1 of EF-G interacts with the L7/12 stalk on the 50S subunit, while domain 4 contacts the shoulder of the 30S subunit in the region where protein S...

one of the most important methods to investigate heavy metals as pollutants is more likely to use bio-monitors which can be applied as an appropriate index to express the quality of environment. in current study, in order to investigate the effects of dominant plants in the desired region and role of aquatic macrophytes in monitoring such elements as zinc (zn), lead (pb), copper (cu) and cadmiu...

The delivery of the diphtheria toxin catalytic domain (DTA) from acidified endosomes into the cytoplasm of eukaryotic cells requires protein-protein interactions between the toxin and a cytosolic translocation factor (CTF) complex. A conserved peptide motif, T1, within the DT transmembrane helix 1 mediates these interactions. Because the T1 motif is also present in the N-terminal segments of le...

BACKGROUND During the translation of mRNA into polypeptide, elongation factor G (EF-G) catalyzes the translocation of peptidyl-tRNA from the A site to the P site of the ribosome. According to the 'classical' model, EF-G in the GTP-bound form promotes translocation, while hydrolysis of the bound GTP promotes dissociation of the factor from the post-translocation ribosome. According to a more rec...