Exposure of monolayer cultures of human urogenital and vaginal (HeLa), human epithelial (HEp-2), normal baboon testicular (NBT), and monkey kidney (Vero) cells to live pathogenic Trichomonas vaginalis resulted in extensive disruption of monolayers. Trypan blue was taken up by all host cells released from cell monolayers, which indicated irreversible damage of these cell types by trichomonads. T...

Class II fumarase sequences were obtained by polymerase chain reaction from five trichomonad species. All residues known to be highly conserved in this enzyme were present. Nuclear run-on assays showed that one of the two genes identified in Tritrichomonas foetus was expressed, whereas no fumarase transcripts were detected in the related species Trichomonas vaginalis. These findings corroborate...

Trichomonas vaginalis infection is the most prevalent nonviral sexually transmitted disease (STD) in the world. A PCR test using vaginal swab samples for the detection of T. vaginalis was developed to add T. vaginalis infection to the growing list of STDs that can be detected by DNA amplification techniques. A primer set, BTUB 9/2, was designed to target a well-conserved region in the beta-tubu...

In this paper we document the role of Phormium tenax as a nurse plant in unimproved pasture. We show that for our study area the regeneration of woody species was limited solely to P. tenax clumps with 22 native and one introduced regenerating woody species present. The number of woody species and of individual woody plants regenerating within P. tenax is not correlated with distance from the e...

26 fowleri, causes a fulminant disease called primary amebic meningoencephalitis (PAM) in an extremely small percentage of people who come in contact with it. As this protozoan tends to inhabit warm, fresh water, most victims acquire the organism while swimming.1 Amebae enter the swimmer’s nose and access the subarachnoid space of the CNS via the olfactory neuroepithelium and olfactory nerves.1...

A new approach of enhancing the adsorption capability of the widely used polymer adsorbent Tenax TA poly(2,6-diphenylene oxide) through its deposition on a nano-structured template is reported. The modified Tenax TA-coated silica nanoparticles (SNP) are incorporated as an adsorbent bed in silicon based micro-thermal preconcentrator (μTPC) chips with an array of square microposts embedded inside...