Buchnericin LB produced by Lactobacillus buchneri LB was purified to homogeneity by a rapid and simple three-step purification procedure including freeze drying, silicic acid adsorption/desorption and cation-exchange chromatography. After the silicic acid and cation-exchange chromatography steps, the activity of buchnericin LB was recovered by 85 and 25%, and its purity increased about 111 and ...

Two new non-barbiturate buffers have been formulated for serum protein electrophoresis on cellulose acetate membranes. Both buffers give five distrinct fractions and are suitable for all systems, but have been primarily designed to meet the needs of the Gelman "Sepratek" system. One buffer is a tris(hydroxymethyl)aminomethane (Tris)/hippurate formulation; the other is composed of Tris/N-[tris(h...

The assay medium contained SO~M-KCI, ZOm~-Tricine, 0.83 mM-EDTA, 1 mMsuccinate, 5 m ~ N a ~ s O ~ , 10 % (w/v) sucrose, 50,u~-carbonyl cyanide p-trifluoromethoxyphenylhydrazone, final pH7.6, in a total volume of 2.0ml. Reaction was started by the addition of chromatophores to give 20,u~-bacteriochlorophyll in assay medium. Release of phosphate was measured colorimetrically after 3 min at 30°C i...

introduction: 99mtc-trodat-1 is a promising new radiopharmaceutical with the potential for routine use as the radiopharmaceutical for dopamine transporters scintigraphy as far as its image quality and daily availability are concerned. here we describe the development of a freeze-dried kit formulation based on the tricine exchange labeling approach for the preparation of this radiopharmaceutical...

The effects of thermal modification wood have been well established, particularly in terms reductions mechanical performance. In recent years, there has an increase studies related to the Maillard reaction. More commonly associated with food chemistry, it involves reaction amines and reducing sugars during cooking procedures. This study attempted combine use modification, subsequent properties ...

UNLABELLED Integrin αvβ6, a member of the integrin family, is specifically expressed in many malignancies but not in normal organs. Overexpression of integrin αvβ6 is usually correlated with malignant potential and poor prognosis. In this study, we describe the synthesis and evaluation of a (99m)Tc-labeled integrin αvβ6-targeting peptide as a SPECT radiotracer for the in vivo imaging of integri...

Subchloroplast particles were prepared by incubation with digitonin and differential centrifugation. Such particles show a high rate of cyclic photophosphorylation but have little or no capacity to form the reversible, light-dependent pH gradient. They also have no activity in the formation of the nonphosphorylated high energy intermediate, in acid-base phosphorylation, and in light-induced abs...