In the light of the difficulties and inadequacies of established methods in the investigation of intestinal malabsorption the report by Stanley and Thannhauser (1949) that radioiodinated olive oil might provide an index of fat absorption appeared to be of considerable interest. Subsequent investigators in America, using a pure neutral fat, triolein, similarly iodinated, have reported on the stu...

A method is presented for measuring lipase activity in serum by use of either triolein or olive oil as substrate. The method requires 3 to 8 mm for each measurement and 5 to 8 mm for initial set-up. The reaction mixture is maintained at the optimum pH of 8.8 by automatic addition of sodium hydroxide (10 mmol/liter) with a ‘pH-Stat.” Amount of sodium hydroxide delivered per unit time reflects li...

The extracellular lipase (LipA) produced by Acinetobacter calcoaceticus BD413 is required for growth of the organism on triolein, since mutant strains that lack an active lipase fail to grow with triolein as the sole carbon source. Surprisingly, extracellular lipase activity and expression of the structural lipase gene (lipA), the latter measured through lacZ as a transcriptional reporter, are ...

Background: Microinjection was tested as a potentially powerful tool to introduce natural and anthropogenic pollutants directly into fish eggs to determine their toxicological impact on fish. With this technique, parental transfer of lipophilic contaminants may be mimicked. Here, we investigated the applicability of pollutant injection into the yolk of early zebrafish (Danio rerio) eggs with sp...

Membrane lipid therapy is a novel approach to rationally design or discover therapeutic molecules that target membrane lipids. This strategy has been used to design synthetic fatty acid analogs that are currently under study in clinical trials for the treatment of cancer. In this context, and with the aim of controlling tumor cell growth, we have designed and synthesized a hydroxylated analog o...

Vitamin A acetate and vitamin A alcohol, triolein I(131), oleic acid I(131), and fat balance tests have been assessed in studies on cases of coeliac disease, pancreatic insufficiency, and some disorders of the small intestinal wall. In coeliac disease a very low serum carotene and flat vitamin A absorption curves have been noted. The contrast between vitamin A acetate and alcohol curves has bee...

Ozonated olive oil was prepared by bubbling ozone-oxygen gas through olive oil until it solidified. Hand C-NMR spectra of the ozonated oil showed that all C-C double bonds in triacyglycerols were converted to ozonide. The spectra also showed no other structural change and the absence of degraded products of ozonide such as aldehydes and carboxylic acids. HPLC chromatogram of the oil showed thre...

Experiments are described showing that an anion exchange resin may be used as a simple means of determining the amount of free radio-activity in emulsions of triolein and oleic acid labelled with (131)I in the form of (131)ICl. In the case of radio-iodinated triolein no special modifications are necessary. With radio-iodinated oleic acid, the working solution is made up with an excess of unlabe...