Stelechocarpus merupakan salah satu genus anggota famili Annonaceae yang tumbuh di kawasan Asia-Pasifik. Sebagai tumbuhan kurang dikenal oleh masyarakat maka perlu adanya upaya konservasi karena jenis mempunyai banyak manfaat bidang farmasi dan kosmetika. Salah strategi mendukung yaitu dengan DNA barcoding masih sedikit informasinya untuk Stelechocarpus. Penelitian ini bertujuan mengevaluasi lo...

BACKGROUND The concept of DNA barcoding for species identification has gained considerable momentum in animals because of fairly successful species identification using cytochrome oxidase I (COI). In plants, matK and rbcL have been proposed as standard barcodes. However, barcoding in complex genera is a challenging task. METHODOLOGY AND PRINCIPAL FINDINGS We investigated the species discrimin...

Methods This study uses maximum-likelihood and Bayesian phylogenetic analyses of chloroplast DNA (atpB–rbcL, trnL–trnF, trnS–trnG, psbA–trnH) and nuclear ribosomal DNA [internal transcribed spacer (ITS) and external transcribed spacer (ETS)] from 24 out of c. 37 ingroup taxa, beast molecular dating, and the dispersal–extinction–cladogenesis method (Lagrange) to reconstruct ancestral geographica...

Three chloroplast regions, rbcL, psbA-trnH and petA-psbJ were applied to assess the genetic relationships among six Curcuma medicinal species, which are difficult to distinguish from morphology. The Maximum Parsimony tree was conducted by Kimura 2-parameter model with MEGA 4. The genetic relationships were linked with geographical distributions among these six species; Curcuma sichuanensis is a...

BACKGROUND Dioscorea is an important plant genus in terms of food supply and pharmaceutical applications. However, its classification and identification are controversial. DNA barcoding is a recent aid to taxonomic identification and uses a short standardized DNA region to discriminate plant species. In this study, the applicability of three candidate DNA barcodes (rbcL, matK, and psbA-trnH) to...

DNA barcodes have been increasingly used in authentication of medicinal plants, while their wide application in materia medica is limited in their accuracy due to incomplete sampling of species and absence of identification for materia medica. In this study, 95 leaf accessions of 23 species (including one variety) and materia medica of three Pharmacopoeia-recorded species of Angelica in China w...

This study proposes the use of genetic analysis as a complementary method for species identification in genus Pinus, particularly cases where anatomical is challenging. Pinus were grouped based on similarities, and efficacy using ycf1b, which most variable identification, rbcL, suggested DNA barcode land plants, was evaluated within each group. Sequences obtained from National Center Biotechnol...

DNA barcoding relies on short and standardized gene regions to identify species. The agricultural and horticultural applications of barcoding such as for marketplace regulation and copyright protection remain poorly explored. This study examines the effectiveness of the standard plant barcode markers (matK and rbcL) for the identification of plant species in private and public nurseries in nort...