Aim: Colourless solutions of mitomycin C (MMC) and 5-fluorouracil (5-FU) are widely used during trabeculectomy to inhibit postoperative scarring. The poor visibility of these agents on the eye has several drawbacks including the inability to accurately assess the area of treatment. This study examined the utility of using trypan blue dye to colour antimetabolites used during trabeculectomy and ...

Resorbing oocytes are heavily stained by trypan blue injected into the haemolymph; this serves as a basis for a quick and convenient method for measuring the degree of resorption. Oocytes in the beginning of their development are most susceptible towards resorptive tendencies. The uptake of trypan blue by normally developing oocytes is proportional to the oocyte surface. From 'double-marker' ex...

Actinobacillus actinomycetemcomitans is a clinically relevant periodontopathogenic Gram-negative coccobacillus that produces a leukotoxin of the RTX cytolysin family. In this study, we evaluated the leukotoxic activity of A. actinomycetemcomitans strains isolated from human and marmosets by Trypan blue exclusion and by the chemiluminescence assays. Among eight A. actinomycetemcomitans human str...

BACKGROUND In the present study, we describe a procedure to cryopreserve the postnatal members of the Leydig cell lineage, including progenitor (PLC), immature (ILC) and adult (ALC) Leydig cells from, respectively 21-, 35- and 90-day-old rats. METHODS The cells were resuspended in a culture medium supplemented with 1% bovine serum albumin (Dulbecco's Modified Eagle's Medium [DMEM]/F12) to a f...

Cancer is a disease in which there is an uncontrolled multiplication and spread, within the body, of abnormal forms of the body’s own cells. Assessment of In-vitro cytotoxicity has been recently become popular as a primary screening method for evaluating anticancer activities of various natural source. This study was carried out to characterize anti-cancer activity of methanolic extract of Mich...

Background Performing accurate sample analyses during the culture of mammalian cells is vital in order to track the number and viability of cells present. This allows operators to recognise when reagents may need to be added to maximise yield, harvest cells appropriately and to identify when a targeted cell concentration has been reached. This is commonly performed via manual cell counts. Cell ...

AIM Colourless solutions of mitomycin C (MMC) and 5-fluorouracil (5-FU) are widely used during trabeculectomy to inhibit postoperative scarring. The poor visibility of these agents on the eye has several drawbacks including the inability to accurately assess the area of treatment. This study examined the utility of using trypan blue dye to colour antimetabolites used during trabeculectomy and t...

There are currently no effective, long-term drug therapies for the treatment of leiomyomas. Pirfenidone (Marnac, Inc.) is an antifibrotic agent that is being tested for use in patients with pulmonary fibrosis. Because leiomyomas are characterized also by increased cell proliferation and tissue fibrosis, we examined the effects of pirfenidone on cell proliferation and collagen expression in cult...

Background The estimation of cell density and cell viability of mammalian cell lines in cell culture has traditionally been performed using the exclusion dye trypan blue that stains “dead” cells when their cell membrane is damaged. In large scale cell cultures using bioreactors this estimation is performed off-line. The online biomass probe is based on the principle that under the influence of ...