The existence of proteins in BALB/c 3T3 cells which interact strongly with trypsin was investigated in order to learn more about how the activities of proteases with trypsin-like specificity might be regulated in mammalian cells. Cell extracts were found to contain 7 to 12 pmol/miUion cells of a protein (TCP) which binds and inhibits trypsin. The trypsin-TCP complex which forms upon incubation ...

Ingestion of a blood meal induces two phases of trypsin synthesis in the midgut of Aedes aegypti females. The first phase, which encompasses the first 4-6 hours following a blood meal, is characterized by the presence of small amounts of early trypsin. The second phase, which occurs between 8 and 36 hours after blood feeding, is characterized by the presence of large amounts of late trypsin. A ...

One of the most significant tasks for proteomic research and industrial applications, is the preparation of recyclable enzyme reactor. Herein, a novel recyclable enzyme reactor has been developed based on monodispersed spherical poly(quaternary ammonium ionic liquid)s particles immobilized trypsin. A new quaternary ammonium ionic liquids functional monomer was first synthesized. The ionic l...

From the ‡European Molecular Biology Laboratory (EMBL) Hamburg c/o DESY, D-22607 Hamburg, Germany, the **Institute of Bioorganic Chemistry, Polish Academy of Sciences, 61–704 Poznan, Poland and Institute of Biochemistry and Molecular Biology, University Hospital, Hamburg-Eppendorf, c⁄o DESY, 22603 Hamburg, Germany, the ¶Laboratoire de Cristallographie et Modelisation des Matériaux Mineraux et B...

A method is described for isolating a crystalline protein of high tryptic activity from beef pancreas. The protein has constant proteolytic activity and optical activity under various conditions and no indication of further fractionation could be obtained. The loss in activity corresponds to the decrease in native protein when the protein is denatured by heat, digested by pepsin, or hydrolyzed ...

One of the main functions alpha-2-macroglobulin (A2M) in human blood serum is binding all classes protease . It known that trypsin , after such interaction, possesses modified proteolytic activity Trypsin first hydrolyzes two bonds A2M’s ‘bait region’, and peptide 705 VGFYESDVMGR 715 released from A2M. In this work, specifics A2M-trypsin interaction were used to determine A2M concentration dire...

Here the relation between digestion of proteins by trypsin covalently bound to paper and in-solution is investigated. The acting on bound. A concentration 0.5% (w/v) results in highest activity all concentrations tested. Additionally, it can be seen that on-paper has retained approx. 50% its activity. Unlike in-solution, stability smart proteolysis samplers was regarded stable for at least four...