This paper characterizes the complexes formed by a small protein, ubiquitin (UBI), and a negatively charged surfactant, sodium dodecyl sulfate (SDS), using capillary electrophoresis (CE), circular dichroism (CD), and amide hydrogen-deuterium exchange (HDX; as monitored by mass spectroscopy, MS). Capillary electrophoresis of complexes of UBI and SDS, at apparent equilibrium, at concentrations of...

background: ubi 29-41 (a derivative of antimicrobial peptide ubiquicidin) labelled with 99mtc is reported to discriminate between bacterial infections and sterile inflammatory processes. in this study, three lyophilized kit were performed, one of them based on the direct labelling with only sncl2 as reducing agent , and other two based on 6- hydrazinopyridine-3-carboxylic acid (hynic) and trici...

An ethnobotanical study was conducted to document local knowledge and potentials of wild edible tubers that has been reported and sighted and to investigate and record their distribution in Pulau Redang and nearby islands of Terengganu, Malaysia. Information was gathered from 42 villagers by using semi-structured questionnaire. These respondents were selected randomly and no appointment was mad...

  Introduction: Ubiquicidin (UBI) 29-41 is a synthetic antimicrobial peptide that binds with the microbial cell membrane at the location of infection. This study was conducted to evaluate its probable efficacy as an infection-imaging agent with potential to differentiate bacterial infection from sterile inflammation in humans. Methods: Fifteen diabetic foot patients ...

Ubiquicidin 29-41 (UBI 29-41) is an antimicrobial peptide with six positively charged residues which preferentially binds to the negatively charged groups present on the microbial membrane by electrostatic interaction at the foci of infection. For this reason UBI 29-41 has been suggested to label by 99m Tc ( 99m Tc-UBI 2941)directly or by using coligand reagents like 6-hydrazinopyridine 3-carbo...

A protein's life inside a cell is usually terminated by the action of proteases that hydrolyse the polypeptide to small peptides or free amino acids. Many proteolytic enzymes are specific for only a few protein substrates, or they mediate highly specialized functions. Promiscuous proteases with broad specificities, however, are generally seques-tered and reside within specialized organelles kno...

Antimicrobial peptides are endogenous antibiotics and effector molecules of innate immunity. These peptides are mainly localised to epithelial linings and circulating neutrophils. They kill microbes by disruption of their membranes. The two main families of mammalian antimicrobial peptides are defensins and cathelicidins. In this thesis, the presence of antimicrobial peptides and proteins in hu...

During past 3 decades, nuclear medicine has flourished as vibrant and independent medical specialty in Iran. Since that time, more than 200 nuclear physicians have been trained and now practicing in nearly 158 centers throughout the country. In the same period, Tc-99m generators and variety of cold kits for conventional nuclear medicine were locally produced for the first time. Local production...

AIM Various studies have been conducted for determining the most optimal method for the early diagnosis of local recurrent or distant metastatic thyroid cancers. The aim of this study was to evaluate the clinical utility of technetium-99m (Tc-99m)-labeled octreotide derivatives in the detection of recurrence or distant metastases in medullary thyroid cancer patients and to compare the lesions w...