The use of accelerated temperature stability studies using the Arrhenius relationship is routine for estimating the stability of a drug at room temperature. Therefore, the acceleration method is used to determine the expiration date of dexamethasone aqueous formulation manufactured in Iran. A high-performance liquid chromatography (HPLC) method utilizing ultraviolet (UV) detection for dexa...

We report a micromethod for the analysis of cyclosporine (CsA), based on quantification of its constituent amino acids. The amino acids were released by gas-phase hydrolysis, derivatized with fluorenylmethyl chloroformate, and separated and analyzed in a reversed-phase HPLC system. The imprecision (CV) of the amino acid analysis was less than 4%, and several determinations of the amount of stan...

This report describes the detailed procedures for Western blot analysis using fluorescent antibodies. After electrophoresis and subsequent electroblotting, the fluorescent-labeled antibodies were visible upon ultraviolet illumination of the polyvinylidene fluoride (PVDF) membranes and could then be photographed to give an accurate record of the blots. Fluorescent labeling allows for photographi...

The major use of alternative light sources (ALS) in the evaluation bloodstains has been primarily focused on detection whole blood, with relatively little attention to visualization blood serum. Serum may become separated from pools during clotting, and because it is invisible certain backgrounds, go undetected by a perpetrator attempting clean up crime scene. Recently, Ultraviolet 365 (UV 365)...

We present a candidate Reference Method for determining the concentration of serum creatinine. The method is based on HPLC combined with enzymatic determination. Creatinine plus 14[C]creatinine is extracted by cation-exchange chromatography, subjected to reversed-phase HPLC, and finally quantified enzymatically. Enzymatic measurement ensures no interference from co-eluting compounds, which has ...

An abscisic acid derivative was formed by reaction with pentafluorobenzyl bromide which allowed highly sensitive detection by gas-liquid chromatography with electron capture detection. In comparison to the methyl ester derivative, the pentafluorobenzyl derivative of abscisic acid was four times more sensitive to electron capture detection and was stable at room temperature in the presence of ul...