Background Cardiovascular MRI benefits from improved SNR-efficiency at ≥3T [1,]2, but is subject to other sources of error, which require careful consideration when transitioning from primary use of 1.5T scanners. For example, chemical shift-induced PC-MRI errors [3] are increased at 3T compared to 1.5T. Chemical shift causes the complex perivascular fat signal to chemically shift into the vess...

Activated macrophages undergo metabolic reprogramming, which drives their pro-inflammatory phenotype, but the mechanistic basis for this remains obscure. Here, we demonstrate that upon lipopolysaccharide (LPS) stimulation, macrophages shift from producing ATP by oxidative phosphorylation to glycolysis while also increasing succinate levels. We show that increased mitochondrial oxidation of succ...

Background: Allergy to Saffron (Crocus sativus) pollen has been described in people involved in processing of saffron flower stamens. Profilins have been identified as a pan-allergen in different plant pollens and foods. This molecule is an actin-binding pro-tein with a molecular weight of 12-16 kDa found in eukaryotic species. Objective: The aim of this study was to generate monoclonal antibod...

The misfolding of the protein α-synuclein is a major contributor to Parkinson’s disease (PD). Three mutations (A53T, A30P and E46K) cause familial PD, and three newly discovered spliced variant forms of the protein (syn-126, syn-112, and syn-98) are also found in many PD patients. Little is known about whether these familial mutants can influence each other’s contributing properties and whether...

The present study provides evidence that rat brain protein kinase C elicits a phosphotransferase activity towards histone and undergoes autophosphorylation in the absence of phosphatidylserine. The tumor pro moter 12-0-tetradecanoylphorbol-13-acetate binds to and activates pro tein kinase C in a phospholipid-free reaction. The apparent activation constant (A1,, = 2.7 IIM) is not modified by the...

Mitochondrial injury contributes to severe drug-induced liver injury. Particularly, mitochondrial permeability transition (MPT) is thought be relevant cytolytic hepatitis. However, the mechanism of MPT unclear and prediction not adequately evaluated in preclinical stage. In a previous study, we found that troglitazone, drug withdrawn due injury, induced via mild depolarization probably resultin...

Galectin-3 is a ß-galactoside-specific lectin implicated in diverse pro cesses involved in cellular interactions. Recently, the Mac-2-binding pro tein, a heavily /V-glycosylated secreted protein with a subunit MTof 97,000, was identified as its ligand. The present study characterizes the interaction between galectin-3 and Mac-2-binding protein in whole cells and measures their relative express...

INTRODUCTION The present experiments were conducted to de termine the carcinogenicity of 2-acetylaminofluorene when it was fed in partially purified diets that varied in casein level between 9 and 60 per cent. Studies involving this broad range of dietary protein level have not been reported previously with this carcinogen. Harris (7) concluded that variations in dietary protein level had no ap...

Single doses of 3SS-labeled reduced glutathione (GSH) were administered intraperitoneally to groups of normal and tumorbearing mice, and radioactivity was determined in the protein and non-protein fractions of blood serum. The results show that GSH is taken up rapidly by the albumin fraction of serum pro tein. Experiments in vitro showed that both mouse and human serum albumin react with GSH in...

OBJECTIVE The mitochondrial uncoupling agent 2,4-dinitrophenol (DNP), historically used as a treatment for obesity, is known to cross the blood-brain-barrier, but its effects on central neural circuits controlling body weight are largely unknown. As hypothalamic melanocortin neuropeptide Y/agouti-related protein (NPY/AgRP) and pro-opiomelanocortin (POMC) neurons represent key central regulators...