Blinatumomab, the bispecific T cell engager, has been demonstrated as the most successful BsAb to date. Throughout the past decade, vector design has great importance for the expression of monoclonal antibody in Chinese hamster ovary (CHO) cells. It has been indicated that expression plasmids based on the elongation factor-1 alpha (EF-1 alpha) gene and DHFR selection marker can be highly effect...

Intergenic regions of polycistronic pre-mRNAs of trypanosomatid protozoans are the sites of two processing reactions: polyadenylation of the upstream gene and trans-splicing of the capped miniexon to the downstream gene. Their close proximity and the lack of consensus motifs at poly(A) sites led us to test whether poly(A) site selection is governed by the location of the downstream splice accep...

abstract one of the most important sources of drinking and agricultural water needs is rivers. one of the oldest methods of getting water from rivers that still has the greatest use is the gravity intake. in this type of intake, due to the disproportionate velocity at the upstream and downstream mouth of intake, occur flow separation and following sedimentation at the upstream mouth. in this st...

This paper investigates the effect of inserting a ring type barrier on leakage flow of brush seals with different bristles clearances (the distance between bristle pack tip and rotor surface). The ring is placed on both upstream and downstream sides of the bristles. An axisymmetric CFD model is employed to calculate radial pressure distribution along backing plate, axial pressure variation on r...

background & aims of the study: heavy metals contaminate drinking water, air and soils. it is very harmful for humans and other live organisms. phytoremediation is one of the direct bio-remediation methods for heavy metal removal from polluted water and soils. this method is accepted environmentally, economically and aesthetically. the purpose of this study was to assess tamarix hispida capacit...

background: this study was carried out to determination of heavy metals (cd, cr, cu, fe, pb, ni and zn) concentration in upstream and downstream of the entry of the wastewater (untreated urban, traditional slaughterhouse waste, leachate from solid wastes has accumulated in river and  run off waters flowing across the city ) to the tembi river. methods: samples were collected for 1 year, before ...