objective: the main purpose of present research was to identify strategic factors influencing to the development of digital libraries in iran.method: research method of this study is grounded theory, which we use systematic method. the study sample consisted of professionals and experts in the field of digital library, and semi-structured interviews used to collect data to achieve theoretical s...

A method is presented to assemble a gene of interest into a linear DNA template with all the components necessary for in vitro transcription and translation in approximately 90 min. Assembly is achieved using a coupled uracil excision-ligation strategy based on USER Enzyme and T4 DNA ligase, which allows the simultaneous and seamless assembly of three different PCR products. The method is suita...

Available online 21 July 2009 Analysis of a survey of the types and extent of tools and techniques related to semi-automatic metadata generation applied in real-world library settings indicates that practical applications in libraries seem to be at an incipient stage. More than half (n=149, 52.5%) of the survey participants (n=285) specify that semiautomatic metadata generation has not been uti...

The engineering of enzymes with altered activity, specificity, and stability, using directed evolution techniques that mimic evolution on a laboratory timescale, is now well established. In vitro recombination techniques such as DNA shuffling, staggered extension process (StEP), random chimeragenesis on transient templates (RACHITT), iterative truncation for the creation of hybrid enzymes (ITCH...

Tandem mass spectral libraries (MS/MS) are usually built by acquiring experimentally measured mass spectra from chemical reference compounds. We here show the versatility of in-silico or computer generated tandem mass spectra that are directly obtained from compound structures. We use the freely available LipidBlast development software to generate 15 000 MS/MS spectra of the glucuronosyldiacyl...

A conceptually new approach to the design of macromolecular receptors for lectins is outlined. Carbohydrate-functionalised Polymer-Scaffolded Dynamic Combinatorial Libraries (PS-DCLs) have been prepared in aqueous solution by the reversible conjugation of carbohydrates possessing acylhydrazide functionalities in their aglycone on to an aldehyde-functionalised polymer scaffold. PS-DCLs have been...