نتایج جستجو برای: using rnease mini kit

تعداد نتایج: 3426840  

Journal: :Molecular pathology : MP 2003
P J Paterson S Seaton J McLaughlin C C Kibbler

BACKGROUND/AIMS Invasive infection with emerging moulds is increasing in incidence and reliable methods for speciating these organisms in tissue sections need to be developed. METHODS Two methods for extracting fungal DNA from paraffin wax embedded tissue sections, based on the TaKaRa DEXPAT kit and QIAamp DNA mini kit, were optimised and compared. DNA was amplified by PCR using pan-fungal pr...

Journal: :Revista da Sociedade Brasileira de Medicina Tropical 2014
Maria Almerice Lopes da Silva Zulma Medeiros Cynthia Regina Pedrosa Soares Elis Dionísio da Silva Demócrito Barros Miranda-Filho Fábio Lopes de Melo

INTRODUCTION Polymerase chain reaction (PCR) may offer an alternative diagnostic option when clinical signs and symptoms suggest visceral leishmaniasis (VL) but microscopic scanning and serological tests provide negative results. PCR using urine is sensitive enough to diagnose human visceral leishmaniasis (VL). However, DNA quality is a crucial factor for successful amplification. METHODS A c...

2014

Samples were collected and stored at −80°C for ≥1 week to avoid the risk of infection with any Echinococcus spp. eggs present. Approximately 250 mg was placed into 2-mL tubes, heated at 65°C for 15 min, and stored at −80°C. The heating and cooling procedure helps to break the parasite egg shells, enabling more efficient DNA extraction. DNA was extracted by using the QIAamp DNA Stool Mini Kit (Q...

2017
Pero Dimsoski

AIM To show that application of the polymerase chain reaction (PCR) method modified for amplification of a low-copy number DNA samples, ie, the isolation of PCR products (IPCRp), would represent improvement in obtaining genotypes from a fecal DNA compared with previously used genotyping methods. METHODS The DNA from the horse fecal matter was extracted by modified Qiagen DNA Stool Mini Kit pr...

Journal: :Journal of clinical microbiology 2004
Ittisak Subrungruang Mathirut Mungthin Porntip Chavalitshewinkoon-Petmitr Ram Rangsin Tawee Naaglor Saovanee Leelayoova

An evaluation of the sensitivities of three DNA extraction methods, i.e., FTA filter paper, a QIAamp stool mini kit, and a conventional phenol-chloroform method, by using specimens with known concentrations of Enterocytozoon bieneusi spores was performed. FTA filter paper and the QIAamp stool mini kit were the most sensitive methods, which could detect E. bieneusi in specimens with a concentrat...

Journal: :Veterinary parasitology 2006
Aaron F Harmon Dante S Zarlenga Michael B Hildreth

A multiplex PCR assay for differentiating strongyle eggs from cattle has recently been described; however, the egg disruption and DNA extraction procedures, though effective, are inadequate for large studies or clinical application. The purpose of this research was to evaluate methods for disrupting trichostrongyle eggs, then assess commercial kits for extracting egg DNA using Ostertagia ostert...

2018
Noah Hull Jonathan Miller David Berry William Laegreid Ashley Smith Callie Klinghagen Brant Schumaker

We compared the performances of various DNA extraction kits for their ability to recover Brucella abortus strain 19 inoculated into Brucella-free bovine tissues. Tissues were homogenized in a FastPrep bead homogenizer and extracted in triplicate by using one of five kits (Qiagen DNeasy, GE Illustra, Omega Bio-tek E.Z.N.A., Quanta Extracta, and IBI Science DNA Tissue kit). Whole blood was also t...

2014
Gro E. A. Strøm Marit G. Tellevik Kurt Hanevik Nina Langeland Bjørn Blomberg

BACKGROUND Few studies comparing multiple methods for DNA extraction from dried blood spots (DBS) on filter paper for PCR targeting the Plasmodium genome have been done. METHODS Frequently-used methods for DNA extraction from DBS using Chelex-100, InstaGene Matrix, QIAamp DNA Mini Kit and TE buffer were compared on a dilution series of a standardized Plasmodium falciparum positive sample. The...

2015
Ingrid E. Jordon-Thaden Andre S. Chanderbali Matthew A. Gitzendanner Douglas E. Soltis

PREMISE OF THE STUDY Here we present a series of protocols for RNA extraction across a diverse array of plants; we focus on woody, aromatic, aquatic, and other chemically complex taxa. METHODS AND RESULTS Ninety-one taxa were subjected to RNA extraction with three methods presented here: (1) TRIzol/TURBO DNA-free kits using the manufacturer's protocol with the addition of sarkosyl; (2) a comb...

2014
Marlo K Sellin Jeffries Andor J Kiss Austin W Smith James T Oris

BACKGROUND This study compared the performance of five commercially available kits in extracting total RNA from small eukaryotic tissue samples (<15 mg). Total RNA was isolated from fathead minnow (Pimephales promelas) tissues (spleen, blood, kidney, embryo, and larvae) using the Qiagen RNeasy® Plus Mini, Qiagen RNeasy® Plus Universal, Promega Maxwell® 16 LEV simplyRNA, Ambion MagMAX™-96 and Pr...

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