Serological prevalence of Leptospira serovars among pigs in Ukraine during the period 2001–2019 | V Ukhovskyi, A Pyskun, L Korniienko, H Aliekseieva, O Moroz, G Kyivska, Mezhenskyi Agricultural Journals

BACKGROUND Equine recurrent uveitis (ERU) is a common cause of ocular pain and blindness in horses. Leptospira spp. have been commonly implicated in the pathophysiology of ERU in mainland Europe and the USA. No recent studies have been carried out in the UK, but Leptospira is reported not to be a major factor in the aetiology of ERU in the UK. OBJECTIVES To establish the prevalence of Leptosp...

OBJECTIVE Detecting antibodies against Leptospira spp. in Neotropical primates and workers in a Colombian Zoo and identifying the risk factors associated with the disease. METHODS A cross-sectional study was performed regarding 65 Neotropical primates and 20 zookeepers. The samples were processed by microagglutination test (MAT) using a reference strain collection consisting of 21 Leptospira ...

Primers for PCR were selected from a sequenced fragment of clone pL590, which contains a repetitive element present in the genome of Leptospira interrogans serovar hardjo type hardjoprajitno (M. L. Pacciarini, M. L. Savio, S. Tagliabue, and C. Rossi, J. Clin. Microbiol. 30:1243-1249, 1992). A specific DNA fragment was amplified from the genomic DNAs of serovar hardjo type hardjoprajitno and nin...

Over 230 serovars of Leptospira interrogans have been identified; however few have been completely characterised. The aim of this study was to characterise the proteome of serovar Canicola and to compare this against the serovars of Copenhageni and Pomona. 2D-LC/MS analysis identified 1653 Leptospira proteins in serovar Canicola; 60 of these proteins were common to Copenhageni and Pomona, 16 of...

leptospirosis is one of the main causes of acute febrile illness and is presumed to be the most widespread zoonotic disease in the world. cattle populations may be infected with serovars hardjo, pomona and grippotyphosa. infection with icterohaemorrhagiae, bratislava, hebdomadis, autumnalis, australis, sejroe, canicola and bataviae serovars also occurs. in this study, 200 serum samples were col...

Blood from patients suspected of leptospirosis 148 specimens were cultured for leptospira. Twenty two specimens were positive (15%). The isolated leptospira were tested against the 24 serovars of standard antisera by Microscopic Agglutination Test (MAT). It was found that all 22 leptospira isolates reacted strongly against L. autumnalis, except 1 isolate that also reacted against serovar djasim...

A newly identified 1 kb DNA fragment amplified by PCR using (AG)8T inter-simple sequence repeats (ISSR) primer and a 631 bp segment of 16S rRNA ribosomal gene amplified by PCR using reported primers were labeled with a alpha32P dCTP for use as DNA probes. These probes were hybridized with DNA extracted from 19 standard pathogenic serovars, 3 standard saprophytic serovars, 33 pathogenic isolates...

Liquid nitrogen freezing is recommended for long-term preservation of Leptospira serovars. However, there is no standard protocol to follow for this methodology. We herein report a simple procedure to preserve well-characterized Leptospira serovars unaltered for long-term storage in liquid nitrogen. Forty-three (43) leptospira strains, cryoprotected with 10% (v/v) glycerol were rapidly frozen i...

Leptospirosis is an important bacterial zoonotic disease that affects humans and many animal species. Knowledge of prevalence of Leptospira in a given geographic region supports the implementation of effective control/prevention programmes and thus reduces the transmission risks. This study provides Leptospira seroprevalence and serovar distribution in dogs and cats on the Caribbean island of S...