The G protein-coupled receptor (GPCR) dimer interface plays an important role in the formation and stabilization of dimer. Therefore, identifying potential receptor-receptor is part studying GPCRs. Various strategies have been employed to study GPCR explore its functional significance, but experimental methods lack robustness calculations are laborious. Herein, we report a combined optimized ca...

Oxytocin and vasopressin are important modulators of a wide variety of social behaviors, and increasing evidence is showing that these neuropeptides are important organizational effectors of later-life behavior as well. We treated day-old gerbil pups with oxytocin, vasopressin, an oxytocin receptor antagonist, a vasopressin V1a receptor antagonist, or saline control, and then measured received ...

Cross-talk between the phospholipase C and adenylyl cyclase signalling pathways was investigated in Chinese hamster ovary (CHO) cells transfected with the V1a and V2 vasopressin receptors. Cell lines expressing V1a, V2, or both V1a and V2 receptors, were established and characterized. Stimulation of V2 receptors by vasopressin induced a dose-dependent increase in cAMP accumulation, whereas stim...

Arginine vasopressin plays an important role in the regulation of social behaviours in rodents. In the Syrian hamster, vasopressin injected directly into the brain stimulates scent marking and aggressive behaviour in a steroid dependent manner and is therefore a useful model for investigating steroid-peptide-behaviour interactions. In this study, we used in situ hybridization and radioligand bi...

The vasopressin-aquaporin 2 system plays a key role in urine concentration in dehydration. In contrast to the upregulation of aquaporin 2, the downregulation of the vasopressin V2 receptor in dehydration is known. We investigated the mechanisms of this downregulation in dehydration using reverse transcription-competitive polymerase chain reaction (RT-competitive PCR) and Western blot analysis. ...

Sato, Yoshihiko, Hiroyuki Hanai, Atsuhiro Nogaki, Koki Hirasawa, Eizo Kaneko, Hisayoshi Hayashi, and Yuichi Suzuki. Role of the vasopressin V1 receptor in regulating the epithelial functions of the guinea pig distal colon. Am. J. Physiol. 277 (Gastrointest. Liver Physiol. 40): G819–G828, 1999.—Vasopressin has a wide spectrum of biological action. In this study, the role of vasopressin in regula...

Vasopressin, also known as arginine vasopressin or antidiuretic hormone, plays a pivotal role in maintaining body homeostasis. Increased vasopressin concentrations, measured by its surrogate copeptin, have been associated with disease severity as well as disease progression in polycystic kidney disease (PKD), and in experimental studies vasopressin has been shown to directly regulate cyst growt...

A plasma membrane fraction was prepared from pig kidney medulla. It contained lysine-vasopressin-sensitive adenylate cyclase activity (maximum activation: 4to 6fold, apparent Km value for the hormone: about 5 X 1OV M). Lysine-vasopressin was not inactivated by the membrane preparation even at low hormonal concentrations. The specific [3H]lysine-vasopressin (vasopressin) binding sites found are ...

Here we discuss the origins of diversity in social behavior by highlighting research using socially monogamous prairie vole. Prairie voles display a rich behavioral repertoire involving pair bonding and consoling that are not observed typical laboratory species. Oxytocin vasopressin play critical roles regulating behavior. receptors show remarkable expression patterns both between within Recept...

Vasopressin and oxytocin are synthesised in the hypothalamus and released to the blood stream via the posterior lobe of the hypophysis. Research during later years has shown that these peptides are also produced in other parts of the brain. The secretion to plasma is stimulated by oestrogen, an effect which is counteracted by progestagen. During delivery the fetus can also produce substantial a...