soybean mosaic virus (smv) which belongs to the virus family potyviridae, causes a disease in soybean that is present in soybean-growing areas of the world, and is widely distributed in northern iran. detection of smv is very important for disease management. in the present study several serological and molecular (nucleic acid- based) methods of rapid virus detection were compared. serological ...

Background and Objective: Viral myocarditis (VMC) is one of the common heart diseases to endanger human health in different age groups, especially children. The aim of this study was to diagnose VMC in children with clinical suspicion of myocarditis (MCI) using multiplex real-time PCR in Birjand Vali-Asr Hospital. Methods: This cross-sectional study was conducted on 19 patients with clinical s...

Background and Aims: Influenza is an acute respiratory illness that is caused by a virus belonging to Orthomyxoviridae family. This virus spreads rapidly every year in cold season and leads to morbidities and mortalities especially in adults and children, which causes billions of dollars of economic losses. Accordingly, development of a rapid, sensitive and inexpensive laboratory diagnosis base...

egg drop syndrome (eds) is caused by a hemagglutinating adenovirus which has become a major cause of lost egg production and sever economic losses in breeder and layer chicken flocks throughout the world. a pcr assay was optimized for detection of eds virus in inoculated allantoic fluids (afs) of duck and chicken emberyonated eggs. two strains of eds viruses were propagated in allantoic cavity ...

background and aims: newcastle disease virus (ndv) is an avian paramyxovirus (a-pmv 1) and one of the major pathogens in poultries. vaccination is intended to control the disease, nevertheless this virus is a growing threat to the poultry industry. so, early detection of the virus can prevent the spread of illness and avoid huge economic losses. towards this goal, in this research, we developed...

methods allplex gi-virus assay was developed to detect pathogens causing viral gastroenteritis, one of the major diseases caused by rna viruses. this one-step multiplex real-time polymerase chain reaction (pcr) based on the mudt technique permits simultaneous amplification and detection of target nucleic acids of norovirus gi, norovirus gii, rotavirus a, adenovirus f, astrovirus, and sapovirus ...

background: watermelon silver mottle virus (wsmov), which belongs to the genus tospovirus, causes significant loss in cucurbitaceae plants. objectives: development of a highly sensitive and reliable detection method for wsmov.materials and methods: recombinant plasmids for targeting the sequence of nucleocapsid protein gene of wsmov were constructed. sybr green i real-time pcr was established a...

  Herpes simplex virus type 1 establishes a latent infection in the peripheral nervous system following primary infection. During latent infection, virus genome exhibit limited transcription, with the HSV LATs consistently detected in latency infected ganaglia. Following ocular infection viral latency develops in the trigeminal ganglia. In this study PCR has been used for detection of HSV-1 nuc...

occupational exposure to blood and body fluids is an important hazard for health care workers, which places them at a high risk for blood-borne infections including hepatitis b virus, hepatitis c virus and human immunodeficiency virus and results in psychological and emotional stresses. several preventive measures have been proposed including pre-exposure ( e.g. , education, use of standard pre...

  Among the viruses infecting honey bees, chronic bee paralysis virus (CBPV) is known to induce significant losses in honey bee colonies. CBPV is an unclassified polymorphic single stranded RNA virus. Using RT-PCR, the virus infections in honey bees can be detected in a rapid and accurate manner. Bee samples were collected from 23 provinces of Iran, between July-September 2011 and 2012. A tota...