Mass vaccination against viral infections such as mumps is a very noticeable and appropriate attempt in common health. Mumps infection has severe complications like deafness, infertility and meningitis. Attenuated live vaccine of mumps is produced and used to prevent the problems. Viral content in the monovalent vaccine is assayed with different methods. Nowadays international standards intro...

A study has been made to establish the statistical significance of results obtained in mouse infectivity titrations of influenza virus. Five titrations, each composed of five replicas, were carried out and 50 per cent end points were calculated for each titration. Three criteria for evaluating the end points were employed, namely, the presence or absence of pulmonary lesions, the occurrence of ...

A plaque technique was found useful for the differentiation and titration of two vaccine strains and a local highly virulent field isolate of Newcastle disease virus.

BACKGROUND Porcine epidemic diarrhea virus (PEDV), a highly pathogenic and transmissible virus in swine, was first detected in the U.S. in May, 2013, and has caused tremendous losses to the swine industry. Due to the difficulty in isolating and growing this virus in cell culture, few vaccine studies using cell culture propagated PEDV have been performed on U.S. strains in pigs. Therefore, the o...

Quantitative PCR and plaque assay are powerful virological techniques used to measure the load of defective or infectious virus in mouse and human. However, these methods display limitations such as cross contamination and long run-time. Here, we describe a novel technique termed as semi-functional quantitative flow cytometry (SFQF) for the accurate estimation of the quantity of infectious lymp...

The previous reports from this laboratory (Yoshino et al., 1956 a, 1956 b) indicated that both mouse passaged CVS and 7-day egg passaged Flury strains of rabies virus could infect the 1-day egg resulting in high viral concentrations in the embryo which were comparable to those usually reached in the infected mouse brain, and that these strains after adapted to the 1-day egg by serial passage co...

A dengue nonstructural protein 1 (NS1) antigen capture enzyme-linked immunosorbent assay (ELISA)-based tissue culture infectious dose-50 (TCID(50)) test (TCID(50)-ELISA) was developed as an alternative to the standard plaque assay for titrating dengue virus. Virus titers obtained by TCID(50)-ELISA were comparable to those obtained by the plaque assay and by the traditional TCID(50)-cytopathic e...

1. Titration and neutralization of the Western strain of equine encephalomyelitis virus can be carried out in vitro by means of tissue culture. 2. The in vitro titration test as presented is a more sensitive method than animal inoculation. 3. Tissue culture may be better than animal inoculation for the detection of small amounts of virus. 4. The neutralization obtained in tissue culture is 100 ...