Objective: To compare the effectiveness of carbon dioxide and carbon dioxide-free incubation system in extracting motile, morphologically normal and chromatin intact sperm populations from the individual semen samples. Materials and Methods: Semen samples were obtained from normozoospermic men presenting for infertility evaluation. Individual samples were divided into two equal aliquots for spe...

Background: Sperm storage via freezing has been useful for men who have difficulty masturbating during assisted reproductive technology (ART) programs and before impotency caused by chemotherapy, vasectomy, and other procedures. Studies were undertaken to evaluate the extent of cryoinjury to both semen and sperm after repeated freezing and thawing in oligozoospermic and normozoospermic samples ...

BACKGROUND Assisted reproductive technology (ART) with washed semen can achieve pregnancy with minimal risk of horizontal and vertical transmission of chronic viral diseases (CVD) such as human immunodeficiency virus (HIV), hepati- tis C virus (HCV) and hepatitis B virus (HBV) among serodiscordant couples. How- ever, few studies have been made of the use made by these couples of ARTs or of the ...

This study was conducted to determine whether pentoxifylline has an in vitro effect on sperm motion characteristics in normozoospermic men. The subjects of the study were 15 male volunteers. After the spermatozoa were washed with mBWW medium, the sperm suspension was divided into two aliquots: one was treated with 1 mg/ml pentoxifylline, the other was used as a control. The sperm motion charact...

This study examined whether washing and preincubation of boar spermatozoa was necessary to achieve high in vitro penetration rates of pig oocytes. In experiment 1, diluted, sperm-rich fractions, stored for 24 h at 16 degrees C (stored diluted sperm) were used. After centrifugation once at 50 g for 3 min, the supernatant was concentrated at 1200 g for 3 min. The pellets were washed (1200 g for 3...

Removal of seminal plasma facilitates stallion sperm survival during storage, but washing may damage chromatin. Therefore, quality was compared in samples following single-layer centrifugation (SLC) or and controls (extension only) two extenders, INRA82 INRA96. Ejaculates from six stallions were split among treatments: SLC, washing, controls, Sperm motility acrosome status evaluated at 0, 24, 4...

s:2086:"Background: Cissampelos capensis, is commonly known by the Afrikaans name "dawidjies" and is the most important and best known medicinal plant of the family Menispermaceae used by the Khoisan and other rural people in the western regions of South Africa. Among numerous other ailments, it is taken to treat male fertility problems. The aim of this study was to investigate the effects of C...

This work was designed to study how this ability is affected by different sperm treatments routinely used for in vitro fertilization (IVF) assay. In this study, boar sperm samples from epididymal or ejaculated origin were processed by three different methods: left unwashed (NW group), washed in Dulbecco's phosphate-buffered saline supplemented with 0.1% BSA (BSA group), and washed on a Percoll(...

OBJECTIVE To test a newly invented intracytoplasmic sperm injection (ICSI) sperm selection method based on sperm hyaluronic acid (HA) binding. DESIGN Comparison of chromosomal disomy and diploidy frequencies in sperm arising from semen and in HA-bound sperm. SETTING Academic andrology laboratory. PATIENT(S) Men presenting for semen analysis. INTERVENTION(S) Washed sperm fractions of 32 ...

BACKGROUND Older men tend to have poorer semen quality and are generally at higher risks for infertility and abnormal reproductive outcomes. METHODS We employed proton-induced X-ray emission (PIXE, 3 MeV proton beam) to investigate the concentrations of zinc, copper, calcium, sulfur, chlorine, potassium, titanium, iron and nickel in washed sperm and seminal plasma from non-smoking groups of 1...