BACKGROUND White spot disease (WSD), a major threat to sustainable aquaculture worldwide, is caused by White spot syndrome virus (WSSV). The diagnosis of WSD relies heavily on molecular detection of the virus by one-step PCR. These procedures are neither field-usable nor rapid enough considering the speed at which the virus spreads. Thus, development of a rapid, reliable and field-usable diagno...

Two epizootics of cultured shrimp occurred in India in 1994. The first case of mass mortalities of tiger shrimp Penaeus monodon in July 1994 was very similar to that caused by yellowhead virus (YHV) infection with regard to gross clinical signs, host species and size of shrimp affected, but it was histologically atypical. Very interestingly, intranuclear inclusions typical of a white spot syndr...

Background Dystrophin, an essential protein functional in the maintenance of muscle structural integrity is known to be responsible for muscle deterioration during white spot syndrome virus (WSSV) infection among prawn species. Previous studies have shown the upregulation of dystrophin protein in Macrobrachium rosenbergii (the giant freshwater prawn) upon white spot syndrome virus (WSSV) infect...

Prevalence status of white spot disease (WSD), caused by white spot syndrome virus (WSSV) and Monodon baculovirus (MBV), was studied in different prawn farms in North and South-24 paragana districts of West Bengal, specifically in the areas previously affected by WSD. In total 283 samples of giant tiger prawn Penaeus monodon, Indian white prawn P. indicus and giant freshwater prawn Macrobrachiu...

White spot syndrome virus (WSSV) is one of the most virulent pathogens causing high mortality in shrimp. In the present study, an open reading frame (termed the p22 gene) was revealed from a WSSV cDNA library. The gene was expressed as a fusion protein with glutathione S-transferase (GST) in Escherichia coli and purified. Specific antibody was raised using the purified fusion protein (GST-P22)....

Viral pathogens appear to exert the most significant constraints on the growth and survival of crustaceans under culture conditions. The prevalence of viral pathogens White Spot Syndrome Virus (WSSV), Hepatopancreatic Parvo Virus (HPV), Monodon Baculo Virus (MBV) and Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV) in Penaeus monodon post-larvae was studied. Samples collected from ...

White spot syndrome virus (WSSV) is the causative agent of white spot syndrome, one of the most devastating diseases in shrimp aquaculture. The genome of WSSV includes a gene that encodes a putative family B DNA polymerase (ORF514), which is 16% identical in amino acid sequence to the Herpes virus 1 DNA polymerase. The aim of this work was to demonstrate the activity of the WSSV ORF514-encoded ...

White spot syndrome virus (WSSV) is one of the most virulent pathogens causing high mortality in shrimp. Herein, the characterization of VP24, a major structural protein of WSSV, is described. When purified virions were subjected to Nonidet P-40 treatment to separate the envelopes from the nucleocapsids, VP24 was found to be present exclusively in the envelope fraction. Triton X-114 extraction ...

White spot syndrome virus (WSSV), the etiological agent of white disease (WSD), is a significant pathogen affecting shrimp farming industry worldwide. generalist mainly infecting decapod crustaceans. The aims this review were to: (1) Re-evaluate and update status reported WSSV host vector species based on methods used when detecting replication transmission to 2) make critical evaluation existi...

A recent study examining genetic variations among the 3 completely sequenced white spot syndrome virus (WSSV) genomes isolated from China, Thailand and Taiwan revealed five major differences among them. Of these differences, a deletion region between ORF 23/24 and a variable region of ORF14/15 prone to recombination were of particular evolutionary significance. Focusing on these regions, 81 WSS...