Nucleotide excision repair (NER) removes a variety of DNA lesions. Using a yeast cell-free repair system, we have analyzed the repair synthesis step of NER. NER was proficient in yeast mutant cell-free extracts lacking DNA polymerases (Pol) beta, zeta or eta. Base excision repair was also proficient without Polbeta. Repair synthesis of NER was not affected by thermal inactivation of the tempera...

Extracts from ‘Zhéri’ and ‘Hicaznar’ varieties of pomegranate, Punica granatum L., were obtained by subjecting powdered peels to extraction using water, water/ethanol (1:1; v/v), ethanol, acetone heptane. Using the agar diffusion assay, extracts with water and/or ethanol shown display significant antimicrobial activity diameters inhibition zones up 20 mm. Ethanolic extracts, which most active, ...

the aim of this study was to evaluate the effects of echinophora cinerea boiss and stachys lavandulifolia vahl extracts on quality and sensory properties of doogh, as a dairy drinking, to enhance public health society. for this purpose, different levels of each plant extract was added to the doogh. stability, acidity, ph, microbial and sensory properties of doogh samples were studied during 45 ...

Assembly of appropriately oriented actin cables nucleated by formin proteins is necessary for many biological processes in diverse eukaryotes. However, compared with knowledge of how nucleation of dendritic actin filament arrays by the actin-related protein-2/3 complex is regulated, the in vivo regulatory mechanisms for actin cable formation are less clear. To gain insights into mechanisms for ...

Eighteen plant extracts from nine traditional Mexican medicinal plants were tested for antifungal activity against two dermatophyte fungal species (Trichophyton mentagrophytes and Trichophyton rubrum), one non-dermatophyte (Aspergillus niger), and one yeast (Candida albicans). The strongest effect was manifested by the hexane extracts from Eupatorium aschenbornianum and Sedum oxypetalum, as wel...

This study was investigated the characteristics of types and main components odor reducing agents used in livestock farms, evaluate efficacy odors pig manure through vitro fermentation. As a result classification according to major components, Microbial & Probiotics (71.3%), Amino acid supplements (11.7%), Composite type (6.1%), Silicate (4.9%), Extract (2.8%), Vitamin (2.0%), Enzyme (1.0%) wer...

Thermal untwisting of DNA is suppressed in vitro in nucleosomes formed with chicken or monkey histones. In contrast, results obtained for the 2 micron plasmid in Saccharomyces cerevisiae are consistent with only 30% of the DNA being constrained from thermal untwisting in vivo. In this paper, we examine thermal untwisting of several plasmids in yeast cells, nuclei, and nuclear extracts. All show...

A simple method for preparing chromatin assembly extracts has not been available for budding yeast. Here I describe such a method in detail. The assembly extract, a crude 100,000g supernatant, is prepared from cells disrupted in a manual or motorized grinder while they are frozen. The core histones and all soluble protein factors required for chromatin assembly under physiological conditions ar...

Stirred cytoplasmic extracts of the yeast Saccharomyces uvarum can, on addition of trehalose, display oscillations in the metabolite concentrations. Unstirred extracts may also develop spatiotemporal structures expressed in the metabolite concentrations. In this paper we present evidence of the existence of such patterns. The latter were recorded using the optical absorption of NADH in an annul...