Yeast aldolase was purified partially by Warburg and Christian (I), who found that it was inhibited by metal-binding agents and could be reactivated by addition of Fe++, Co++, or Zn++ ions. Later, this enzyme was further purified, treated with a chelating agent, and crystallized as a mercury salt (2). In this form, the enzyme was still active when tested in the presence of cysteine and Zn++ ion...

The inoculation of a Debaryomyces hansenii strain in dry fermented sausages with reduced fat and salt contents was evaluated in terms of chemical, microbial and consumer acceptability. The implantation of the inoculated yeast strain was confirmed by RAPDs of M13 minisatellite. A reduction of 17e20% salt and 10e16% fat content was achieved. These reductions affected the sausage quality by produc...

During fermentation for ethanol production, yeasts are subjected to different kinds of physico-chemical stresses such as: initially high sugar concentration and low temperature; and later, increased ethanol concentrations. Such conditions trigger a series of biological responses in an effort to maintain cell cycle progress and yeast cell viability. Regarding osmostress, many studies have been f...

The global yeast autolysates and extracts industry is expanding dynamically, with annual market growth rates projected at over 8% from 2009 to 2015. Market drivers include the rapid growth of the processed food market. One of the main uses of yeast extract is as a natural ingredient for savory flavor creation and flavor enhancement, salt replacement, and nutritional profile enhancement of proce...

High soils salinity is a main factor affecting agricultural production. Studying the function of salt-tolerance-related genes is essential to enhance crop tolerance to stress. Rab7 is a small GTP-binding protein that is distributed widely among eukaryotes. Endocytic trafficking mediated by Rab7 plays an important role in animal and yeast cells, but the current understanding of Rab7 in plants is...

Plant vacuolar NHX exchangers play a significant role in adaption to salt stress by compartmentalizing excess cytosolic Na+ into vacuoles and maintaining cellular homeostasis and ionic equilibrium. We cloned an orthologue of the vacuolar Na+/H+ antiporter gene, VrNHX1 from mungbean (Vigna radiata), an important Asiatic grain legume. The VrNHX1 (Genbank Accession number JN656211.1) contains 2095...