نتایج جستجو برای: zinc finger nuclease
تعداد نتایج: 105645 فیلتر نتایج به سال:
Zinc-finger nucleases (ZFNs) have been successfully used for rational genome engineering in a variety of cell types and organisms. ZFNs consist of a non-specific FokI endonuclease domain and a specific zinc-finger DNA-binding domain. Because the catalytic domain must dimerize to become active, two ZFN subunits are typically assembled at the cleavage site. The generation of obligate heterodimeri...
Zinc-finger nucleases can be designed to cleave arbitrary genomic sequences, which stimulates localized mutagenesis and targeted gene replacement. Both outcomes depend on normal cellular processes of DNA repair, and this study examines the pathways responsible. Gene replacement by homologous recombination depends on the activity of the Rad51 protein. Much of local mutagenesis depends on DNA lig...
Colicin E7 is a natural bacterial toxin. Its nuclease domain (NColE7) enters the target cell and kills it by digesting the nucleic acids. The HNH-motif as the catalytic centre of NColE7 at the C-terminus requires the positively charged N-terminal loop for the nuclease activity-offering opportunities for allosteric control in a NColE7-based artificial nuclease. Accordingly, four novel zinc finge...
foxP2, a forkhead-domain transcription factor, is critical for speech and language development in humans, but its role in the establishment of CNS connectivity is unclear. While in vitro studies have identified axon guidance molecules as targets of foxP2 regulation, and cell culture assays suggest a role for foxP2 in neurite outgrowth, in vivo studies have been lacking regarding a role for foxP...
The Rockefeller University Press $30.00 J. Cell Biol. Vol. 205 No. 5 721–735 www.jcb.org/cgi/doi/10.1083/jcb.201403041 JCB 721 A. Grassart and A.T. Cheng contributed equally to this paper. Correspondence to David G. Drubin: [email protected] Abbreviations used in this paper: ACTB, -actin; CCP, clathrin-coated pit; CCS, clathrin-coated structure; CME, clathrin-mediated endocytosis; CLTA, clat...
We describe a fundamentally novel feat of animal genetic engineering: the precise and efficient substitution of an agronomic haplotype into a domesticated species. Zinc finger nuclease in-embryo editing of the RELA locus generated live born domestic pigs with the warthog RELA orthologue, associated with resilience to African Swine Fever. The ability to efficiently achieve interspecies allele in...
Clinical scale zinc finger nuclease (ZFN)-driven gene-editing of PD-1 in tumor infiltrating lymphocytes (TIL) for the potential treatment of metastatic melanoma Joal D Beane, Gary K Lee, Zhili Zheng, Nimisha Gandhi, Daniel Abate-Daga, Mini Bharathan, Mary Black, Matthew Mendel, Zhiya Yu, Sadik H Kassim, Smita Chandran, Martin Giedlin, Dale Ando, Ed Rebar, Andreas Reik, Michael Holmes, Philip D ...
نمودار تعداد نتایج جستجو در هر سال
با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید