Polyacrylamide gel electrophoresis (PAGE) is a common technique used in Biochemistry for separation of macromolecules [I]. It is particularly useful to ascertain the degree of protein purity and confirm molecular masses obtained from chromatographic methods [2-51. In addition PAGE can be developed as zymogram (native gel) to detect the presence of enzyme activity [6]. In this report we describe...

Proteases are involved in the regulation of many biological functions. This study describes a novel method for detecting protease activity by fluorescent zymogram in-gel protease assays, using SDS polyacrylamide gels copolymerized with a peptide-MCA (4-methyl-coumaryl-7-amide) substrate. This method allows simultaneous determination of protease cleavage specificity and molecular weight. Trypsin...

Zymogram studies of peptide hydrolases from the human intestinal brush border and cytoplasmic fractions produced multiple bands--that is, up to seven--while the brush border membrane produced only a single band of enzyme activity. With all of the substrates tested except L-leucyl-L-leucyl-L-leucine, a band having anodic mobility identical with that produced by the brush border enzymes was produ...

A large number of wheat recombinant lines were analysed for storage protein (glutenins) and active protein (betaamylase) polymorphism, using electrophoresis. The purpose of this paper was to outline the relationships between proteinogram types and bread making quality as well as between zymogram types and embryogenic potential. The experimental results emphasized that a great majority of the re...

The alcohol dehydrogenase zymogram phenotypes of 40 wheat strains, each possessing a distinctive chromosomal constitution, were determined. Genes involved in the production of alcohol dehydrogenase were located on the beta arm of chromosome 4A and on chromosomes 4B and 4D. The results support the hypothesis that the active alcohol dehydrogenase isozymes are dimers composed of the six possible c...