The small thiol β-mercaptoethanol (BME) has been used as an anti-blinking reagent for CdSe/ZnS quantum dots (QDs), although its effects on QD photoluminescence are complex. It acts as an antioxidant as well as a hole scavenger on both CdSe and CdTe, which leads to changes in emission intensity and lifetime that vary qualitatively according to BME concentration, time of incubation, and pH of the...

Aspergillus terreus FBCC 1369 was grown in solid-state culture under statistically optimized conditions. β-Mannanase was purified to apparent homogeneity by ultrafiltration, anion exchange and gel filtration chromatography. A purification factor of 10.3-fold was achieved, with the purified enzyme exhibiting specific activity of 53 U/mg protein. The purified β-mannanase was optimally active at p...

Molecular markers are important tools in the characterization of plant genetic diversity and can provide support for conservation strategies endangered populations. The different molecular techniques involve evaluation many individuals; therefore, it is crucial to have fast, efficient, inexpensive methods DNA extraction. Given importance Aroeira (Myracrodruon urundeuva Fr. All.) pertinent optim...

Identical amounts of each protein were analyzed by SDSPAGE and with the Experion system (Bio-Rad Laboratories). For SDS-PAGE, samples were prepared by mixing equal volumes of protein sample and 2x Laemmli sample buffer with or without 5% β-mercaptoethanol. Following separation on CriterionTM Tris-HCl 4–20% precast gels, gels were stained with Bio-SafeTM colloidal Coomassie Blue stain and imaged...

Thermoactive xylanases have important applications in the industrial deconstruction of lignocellulosic plant biomass, due to their sustained activity even at high temperature conditions bioreactors. We herein report development a thermoactive xylanolytic microbial consortium from semi-digested contents goat rumen and characterization enzyme cocktail produced by it. The exhibited maximum endoxyl...

1. The reduction of NAD(+), by an enzyme preparation from rat liver, in the presence of 2-mercaptoethanol is reported. 2. It is suggested that the NAD(+)-linked alcohol dehydrogenase in the extract transfers hydrogen from 2-mercaptoethanol to NAD(+). 3. Both yeast and horse-liver alcohol dehydrogenases were observed to reduce NAD(+) in the presence of 2-mercaptoethanol. 4. Some interactions of ...

Submit Manuscript | http://medcraveonline.com Abbreviations: HR: Hypersensitive Response; SAR: Systemic Acquired Resistance; WIPK: Wound-Induced Protein Kinase; SIPK: Salicylic Acid-Induced Protein Kinase; TCA: Trichloroacetic Acid; 2-ME: 2-Mercaptoethanol; SDS: Sodium Dodecyl Sulphate; PMSF: Phenyl Methyl Sulfonyl Fluoride; 2DE: 2D Gel Electrophoresis; DTT: Dithiothreitol; aact: Acetyl-CoA C-A...

Abbreviations: GMP: Good Manufacturing Practice; BM: Bone Marrow; HSC: Hematopoietic Stem Cells; MSC: Mesenchymal Stem/Stromal Cells; HCT: Hematopoietic Cell Transplantation; NK: Natural Killer; NOD/SCID: Non-Obese Diabetic/Severe Combined Immunodeficient mice; aGVHD: acute Graft Versus Host Disease; LTCG: Laboratory of Cell and Gene Therapy; EBMT: European group for Blood and Marrow Transplant...

Stachybotrys microspora is a filamentous fungus secreting multiple β-glucosidases. Two of them were characterized. The third one, named bglG, was also characterized and used for various investigations. The current work undertakes the plausible role played by some cultural conditions and physico-chemical properties to improve bglG time course synthesis and also its catalytic efficiency. Indeed, ...