نتایج جستجو برای: استخراج igy
تعداد نتایج: 43756 فیلتر نتایج به سال:
Limited antifungal drugs, their high toxicity, and the emergence of resistant Candida albicans strains indicate the need for novel therapeutic strategies. The objectives of this study were to prepare antibodies against C albicans in an egg-laying hen and evaluate the efficacy of these antibodies in preventing the dissemination of C albicans in BALB/c mice. An egg-laying hen was immunized with a...
We obtained rabies-specific egg yolk antibodies (IgY) by immunizing hens with recombinant His-tagged nucleoprotein and phosphoprotein (rN, rP) of the rabies virus (CVS-11 strain) expressed in Escherichia coli. The anti-rN and rP IgY were shown to bind specifically to the respective proteins of the CVS-11 strain of rabies virus by Western blotting, immune fluorescent assay and immunohistochemist...
Following genetic immunization of laying ducks with a plasmid expressing Helicobacter pylori UreB (large subunit of urease), IgY against UreB were obtained from egg yolks. These polyclonal and monospecific IgY antibodies are of higher-titer and specifically recognize recombinant H. pylori urease purified from Escherichia coli. To our knowledge this is the first report describing generation of I...
Effects of egg york containing IgY specific for Helicobacter pylori on the bacterial growth and intragastric infection were investigated in comparison with a proton-pump inhibitor pantoprazole. For in vitro anti-bacterial activity test, H. pylori (1×10(8) CFU/mL) was incubated with a serially diluted IgY for 3 days. As a result, IgY fully inhibited the bacterial growth at 16 mg/mL, which was de...
BACKGROUND Pandemic influenza poses a serious threat to global health and the world economy. While vaccines are currently under development, passive immunization could offer an alternative strategy to prevent and treat influenza virus infection. Attempts to develop monoclonal antibodies (mAbs) have been made. However, passive immunization based on mAbs may require a cocktail of mAbs with broade...
Background The study aimes to determine the effect of administering anti-T. gondii membrane IgY against liver damage (Necrotic index) and the effectiveness of the antibody's delivery time. Materials and Methods This research was a laboratory experiment with five treatments and five replicantions. Each treatment used female mice (Mus musculus) as animal models. The treatment groups consisted o...
The protective effect of immunoglobulins derived from chicken egg yolk (IgY) against infection by Canine parvovirus 2 (CPV-2) was evaluated in 10 beagle dogs orally challenged with a strain of the virus. The 2-mo-old dogs were divided into 3 groups and treated with powders containing CPV-2 IgY or normal egg yolk for 7 d after the challenge. The 4 dogs receiving normal egg yolk (control group) d...
BACKGROUND Toxoplasma gondii may cause abortions, ocular and neurological disorders in warm-blood hosts. Immunized mammals are a wide source of hyperimmune sera used in different approaches, including diagnosis and the study of host-parasite interactions. Unfortunately, mammalian antibodies present limitations for its production, such as the necessity for animal bleeding, low yield, interferenc...
Chinese Cobra (Naja naja atra) bite is one of the leading causes of snake-bite mortality in China. The traditional anti-cobra venom serum therapy was found to be expensive and with high frequency of side effects. Therefore attempts were made to generate a high titer immunoglobulin from egg yolk (IgY) of crude cobra-venom immunized Leghorn hens, and to standardize an effective method for produci...
The focus of this report was to assess the role of passive immunization using immunoglobulins derived from chicken egg yolk in protection against M. gallisepticum infection in chickens. Immunoglobulins were produced by egg yolk technology. ELISA was used to evaluate their immunoreactivity and western immunoblotting demonstrated that chicken egg yolk immunoglobulins Y (IgY) were capable of recog...
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