NBS-LRR-type disease resistance gene-like cDNA, induced by salicylic acid (SA) was cloned from rye Secalecereale L. (2n = 14RR) var. Petkus, which has rust genes such as Lr26, Sr31 andRr9. We designed primers based on the NBS region and performed PCR using Petkus genomic DNA a template. Next, we TA-cloned 532-bp fragment containing five homologous amino sequences in region. The SA-treated showe...

در این مقاله سیستم تأمین انرژی و تخلیه جریان سیم‌­پیچ چنبره‌ای توکامک دماوند به منظور افزایش زمان میدان‌های مغناطیسی با هدف ماندگاری پلاسما، طراحی شبیه‌سازی شده است. حال حاضر سیم­‌پیچ دارای نیم سیکل سینوسی مدت ms 100 پیک kA 12 است که میدان مغناطیس تقریباً T 1/1 مرکز چنبره تولید می‌کند ناحیه تخت آن حدود 20 می‌باشد برای تشکیل محصورسازی پلاسما استفاده می‌گردد. ارتقاء 200، ضروری سیستم‌های کنترل مربو...

interferon regulatory factor 7 (irf7) gene was cloned from a subtractive cdna library constructed with mrnas obtained from rainbow trout (oncorhynchus mykiss) macrophage cell line (rts-11). using expressed sequence tag clones of submitted irf7 amino acid sequences, specific primers were designed. results showed that irf7 cdna contains an orf of 1251 nucleotides that translates into a 416 residu...

background: because of the strong immunologic responses of surface protein tasp in theileria annu­lata infected host, we tried to characterize this protein in a t. annulata isolate from iran. methods: the rna prepared from t. annulata infected cells was used to produce smart-ds-cdna. the double strand cdna was then amplified with primers derived from tasp mrna se­quences. the pcr product was cl...

There is increasing interest in interfacing dissociated neuronal cultures with planar multielectrode arrays (MEAs) for the study of the dynamics of neuronal networks. Here we report on the successful use of three-dimensional tip electrode arrays (3D MEAs), originally developed for use with brain slices, for recording and stimulation of cultured neurons. We observed that many neurons grew direct...