One limitation of the widely used RNA-seq method is that long transcripts are represented by more reads than shorter transcripts, resulting in a biased estimation of expression levels. The 3' RNA-seq method, which yields only one sequence per transcript, bypasses this limitation. Here, RNA was extracted from two samples, in which we expected to find differentially expressed genes. Each was proc...

Background: The stress is one of main factors effects on production system. Several factors (both genetic and environmental elements) regulate immune response to stress. Objectives: In order to determine the major immune system regulatory genes underlying stress responses, a learning Bayesian network approach for those regulatory genes was applied to RNA-...

هدیکچ هقباس و فده : ناطرس زا بسانم تاعلاطا اب یم صاخ یئایفارغج هیحان رد فلتخم یاه همانرب ناوت ار یبط یاه یارب لابرغ و نامرد یرگ ) Screening ( هورگ دومن صخشم رطخرپ یاه . نآ زا هک اج گرم نازیم نانمس ناتسا تشادهب زکرم لااب ریم و ی زکرم رد یناقوف شراوگ هاگتسد ناطرس رثا رب ار ی ا هدرک شرازگ ناریا تس . یماـمت تـبث و یـسررب یارـب تفریذپ ماجنا یعماج قیقحت نانمس ناتسا یموب تیعمج نیب رد ناطرس دراوم ...

كچ ي هد :فده و هقباس و ينميا رب رچسوپ لرتنك رد للاتخا تيلاعف ماگنه رد تاكرح تيفيك اه ي و هرمزور ريرايا ييشزرو يم رب رروم لماوع زا يكي .دراذگ رچسوپ لرتنك فاطعنا يم تلاضع يريذپ يلع .دشاب اتتيسن عويش مغر يهاياوك داييز ه رد ،گنيرتسمه هلضع ي چ هعلاطم طاتارا يا ن يسررب لداعا اب هلضع نيا يهااوك هديش هيعلاطم نييا زا فديه .تيسا و كيااتسا لداعا اب گنيرتسمه هلضع يهااوك طاتارا يسررب يم ملاس...

ةیتوبكنعلا ةكبشلا و ينورتكللإا ثدحتلا و دیربلا لثم ةددعتملا اھتامدخب تینرتنلإا ةكبش ربتعت لا تامولعملا لدابت و لقنل ةیلاعف رثكلأا ةقیرط . يتلا تایوتحملاب ةمحدزم تدغ ةكبشلا هذھ نأ لاإ امداصت تببس " احضاو " امداصت لب اندیلاقت و انتاداع عم " نوناقلا عم . ةلیلق و ةئیذبلا عقاوملا ةیرصنعلا و ةیھاركلا ىلع عجشت يتلا عقاوملا و ةیحابلإا تایوتحملا تاذ ماشتحلاا لاإ تسیل يھ لافطلأا مھنمض نم و تینرتنلإا ةكبش...

RNA-Seq has emerged as a revolutionary technology for transcriptome analysis. In this article, we report a systematic comparison of RNA-Seq and high-density exon array for detecting differential gene expression between closely related species. On a panel of human/chimpanzee/rhesus cerebellum RNA samples previously examined by the high-density human exon junction array (HJAY) and real-time qPCR,...

Sequencing of RNA (RNA-Seq) was invented approximately 1 decade ago and has since revolutionized biological research. This update provides a brief historic perspective on the development of RNA-Seq and then focuses on the application of RNA-Seq in qualitative and quantitative analyses of transcriptomes. Particular emphasis is given to aspects of data analysis. Since the wet-lab and data analysi...

Rapidly growing public gene expression databases contain a wealth of data for building an unprecedentedly detailed picture of human biology and disease. This data comes from many diverse measurement platforms that make integrating it all difficult. Although RNA-sequencing (RNA-seq) is attracting the most attention, at present, the rate of new microarray studies submitted to public databases far...

کچ ی هد هکبش هکبش رگسح یاه رگسح زا یا هنیزه مک و دودمح یژرنا اب ،کچوک یاه دنتسه یا یم شخپ رظن دروم هقطنم رد هک هداد ات دنوش عجم طیمح زا ار ییاه شزادرپ یارب و هدونم یروآ یزکرم هرگ هب ١ دننک لاسرا . م نینچهم ی هب و هداد صیخشت طیمح رد ار یدادخر عوقو دنناوت دنناسرب یزکرم هرگ علاطا . هکبش هنوگنیا ریخا یالهاس رد هنیمز رد اه یاهدربراک فلتمخ یاه هدرک ادیپ یدایز دنا . زا دنترابع اهدربراک نیا هلجم زا...

RNA-sequencing (RNA-seq) allows global gene expression analysis at the individual transcript level. Accurate quantification of transcript variants generated by alternative splicing (AS) remains a challenge. We have developed a comprehensive, nonredundant Arabidopsis reference transcript dataset (AtRTD) containing over 74 000 transcripts for use with algorithms to quantify AS transcript isoforms...