Expression of eukaryotic proteins in E. coli often results in their aggregation. Proper folding and solubility of therapeutical proteins are the pre-requisite for their bioactivity. This is not achieved in cytoplasmic expression in E. coli because of the absence of disulfide bonds formation. A novel expression/secretion vector was constructed which exploited β-lactamase signal sequence to trans...

BACKGROUNDS Epidermal growth factor (EGF) is a 53 amino acid polypeptide and its receptor EGFR is an established therapeutic target for anti-tumor therapy. Two major categories of EGFR-targeted drugs include monoclonal antibodies (mAbs) and small molecular tyrosine kinase inhibitors (TKIs). However, drug resistance occurs in a significant proportion of patients due to EGFR mutations. Since EGFR...

Among the various human growth factors, epidermal growth factor (hEGF, consisting of 53 amino acids) has various effects on cell regeneration, stimulation of proliferation, migration of keratinocytes, formation of granulation tissues, and stimulation of fibroblast motility, which are important for wound healing. Owing to their multiple activities, EGFs are used as pharmaceutical and cosmetic ag...

BACKGROUND Epidermal growth factor (EGF) plays a fundamental role in the healing of wounds relating to skin damage, the cornea, and the gastrointestinal tract. OBJECTIVES The aim of this study is the cloning, expression, and purification of recombinant human EGF (rhEGF), and an assessment of its activity. MATERIALS AND METHODS In the present experimental study, a synthetic pET28a (+) -hEGF ...

background and objective: use of elastin-like proteins (elps) provides high-performance protein purification without need for chromatography. in line with cost reduction and facilitation of recombinant proteins purification, which represent a high percentage of production costs, in this project, we eliminated the need for proteases in the process of separation of recombinant proteins from elp b...

Aquaporins (AQPs) are a family of small, integral membrane proteins that have been shown to play an important role in tumor development and metastasis. Several studies have demonstrated that expression of AQP3 contributes to the enhanced migration of epithelial cells and is related to differentiation, metastasis and vascular invasion in lung and gastric cancer. Therefore, we investigated whethe...

The onshore oil and natural gas industries of China have started a large-scale development when crude oil reserves have been difficult to recover. The stratum fracture modification is an indispensable measure to efficiently develop oil and gas fields. Hydraulic fracturing is the most important reservoir stimulation technique, but it is still faced with various problems such as the failure to fr...

expression of eukaryotic proteins in e. coli often results in their aggregation. proper folding and solubility of therapeutical proteins are the pre-requisite for their bioactivity. this is not achieved in cytoplasmic expression in e. coli because of the absence of disulfide bonds formation. a novel expression/secretion vector was constructed which exploited β-lactamase signal sequence to trans...

For mass production of human epidermal growth factor (hEGF), silkworm baculovirus expression vector system (BEVS) was adopted in this study. hEGF gene was in-frame fused with polyhedrin (Ph) gene under the control of Ph promoter and was used to co-transfect BmN cell with the modified Bombyx mori baculovirus DNA to obtained recombinant virus. The ELISA showed a maximum expression on day 4 in lar...