A method is described by which complement fixation is detected with an enzyme linked immunosorbant assay (ELISA) technique. The method obviates the need for sensitised sheep red blood cells as an indicator of complement fixation and the titration of complement is not critical. The dose response curve has the advantage of being steep and the test result is read photometrically. As test serum and...

The knowledge that exists of the role of complement in immune reactions has been gained from investigations limited almost entirely to studies in vitro. Very little is known of the role of complement in immune reactions in vivo. I t has not been demonstrated that complement fixation occurs in animal tissues in vivo in some way analogous to the fixation of complement to antigen-antibody aggregat...

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Full blood count and erythrocyte sedimentation rate, antistreptolysin titre, albumin/ globulin ratio, blood sugar estimation, gonococcal complement-fixation test, Wassermann and Treponema pallidum immobilization tests, Waaler-Rose latex-fixation test, Toxoplasma complement-fixation test, Tine test, histoplasmosis complement-fixation test. Urine and stool examination for parasites and bacteria. ...

A micro complement fixation assay was devised to measure the type-specificity of anti-M antibody. Unabsorbed sera were from rabbits hyperimmunized with heat-killed streptococci and from humans with naturally occurring antibody or immunized with purified M protein vaccines. These sera fixed complement only in the presence of homologous M proteins (serotypes 1, 3, 6, 12, and 14). The complement f...