به طور کلی، هر سلول طبیعی بدن انسان، که مشتمل بر قسمت‌های مختلفی همچون هسته، غشاء سلولی و نانوالیاف پروتئینی می‌باشد، دارای سختی استحکام مکانیکی مشخصی است. هرگونه تغییر محسوس در این استحکام، نشان از یک بیماری دارد. یکی راه‌های تشخیص بیماری‌های مختلف سرطان های سنجش میزان تغییرات رخ داده خواص آن‌ها می‌باشد. مقالۀ حاضر، مشخصات شامل مدول الاستیسیته نیروی چسبندگی Ago-1522 سلول‌های پوست محسوب می‌شود،...

Biofilm cells typically exhibit increased resistance to antimicrobial agents and the host immune system. In vitro antibiofilm activity has been studied widely in the present’s days. It has become necessary to apply various microscopic techniques in order to evaluate in situ anti-biofilm activity and ultrastructural changes. A wide variety of methods can be applied for visualization and quantifi...

In cazul anumitor tipuri de investigatii prin microscopie pot fi obtinute rezultate doar daca operatiile aferente sunt executate avand ca suport imgini cu o focalizare uniforma. Una dintre tehnicile prin care se pot obtine astfel de reprezentari este fuziunea imaginilor. In acest articol prezentam doua metode de fuziune a imaginilor bazate pe operatorul Sum-modified Laplacian (SML) care realize...

Reactive elements (RE), such as Ce, La or Y, are known to improve oxidation resistance of Fe based alloys that form Cr2O3 scales. The current investigation aims to characterize the oxide scale in a Fe-22 wt.% Cr alloy containing 120 ppm La and 270 ppm Ce (added during melt-stage processing) as a function of oxidation times (at 800°C in dry air) during the transient stage of scale formation. The...

Nowadays there are no doubts about the positive impact that fruits and vegetables have on human health. Plant fibres (i.e. non-digestible carbohydrates) are widely present in daily food, such as soups, dressings and sauces. On the other hand, agricultural (non-wood) by-products have a low economical value. However, those residues are frequently important sources of fibres so an important resear...

Composite materials with unique architectures are ubiquitous in nature, e.g., marine shells, sponge spicules, bones, and dentine. These structured organic-inorganic systems are generated through self-assembly of organic matter (usually proteins or lipids) into scaffolds, onto which the inorganic component is deposited in organized hierarchical structures of sizes spanning several orders of magn...

The effects of the N-formyl methionyl peptide, formyl-methionyl-leucyl phenylalanine (fMLF) on the lateral mobility of the complement receptor type 1 (CR1/CD35) in glass-adherent human neutrophils were investigated, using fluorescence recovery after photobleaching (FRAP) and confocal microscopy (CSLM). It was found that addition of 0.1-1 microM fMLF increased the diffusion constant (D) of CR1/C...

Abstract The cyclic growth and shrinkage of solid oxides ( i.e. wollastonite) in $$\text {CaO}$$ CaO – {SiO}_2$$ SiO 2 -based slags is investigated in-situ by means High-Temperature Confocal Scanning Laser Microscopy (HT-CSLM). compositions ...

This paper presents a fast method for enhancing the images of the vascular network of zebrafish embryo from Confocal Scanning Laser Microscope (CSLM) stained with fluorescent probe. Specifically, we split the 3D enhancement process into two separate stages and adopt different methods respectively to deconvolve the lateral and optical directions in the observed images. The resulting algorithm in...

This unit describes fluorescence recovery after photobleaching (FRAP) and fluorescence loss in photobleaching (FLIP) using commercially available confocal scanning laser microscopy (CSLM). Photobleaching is the photo-induced change in a fluorphore that abolishes that molecule's fluorescence. The different characteristics of green fluorescent protein (GFP) chimeras in a cell can be studied by FR...