quinolones are broad-spectrum antibacterial agents.they have many clinical uses which are increasing.quinolones exert antibacterial activity primarily by inhibiting bacterial DNA gyrase.the inhibition of DNA gyrase by the quinolones are greatly influenced by the nature of the C-7 substituent on the quinolones molecule.substitution of bulky functional groupd is also possible in C-7 position.furt...

Sonicated unilamellar lipid vesicles, consisting of egg lecithin, stearylamine, and cholesterol in 7:2:1 molar ratios, promoted the aggregation of tobacco (Nicotiana glutinosa) protoplasts with the aid of mono- or divalent cations.A reaction mixture containing liposomes (0.4 micromoles lipid per milliliter), 50 millimolar CaCl(2), 0.5 molar mannitol, and 5 x 10(5) protoplasts per milliliter res...

1 hemorrhagiC dm white-tailed dee] virus serotype 2 a positive contra deer with viremi milliliter of bloOO on deer with viR of EHDV-2 inge lahillei. TIters of 10glO 2.7 TCIDsc: ipennis. This rep lahillei may be a

Cancer stem cells (CSCs) are a subset of cancer cells in tumors or established cancer cell lines that can initiate and sustain the growth of tumors in vivo. Cancer stem cells can be enriched in serum-free, suspended cultures that allow the formation of tumorspheres over several days to weeks. Brefeldin A (BFA) is a mycotoxin that induces endoplasmic reticulum (ER) stress in eukaryotic cells. We...

A previously described method for determination of hyaluronic acid is modified for the analysis of pleural and ascitic fluids. After 20 microL of the acellular supernate is precipitated with ethanol, the precipitate is digested overnight with chondroitinases and chondroitin sulfatases. The content of hyaluronic acid-derived disaccharide is then analyzed by "high-performance" liquid chromatograp...

Filter paper discs impregnated with solutions containing 0.25, 0.5 and 1 mg/milliliter of thionin acetate, 0.75 and 1.5 mg/milliliter of basic fuchsin and 0.5 mg/milliliter of thionin blue were used in the typing of Brucella species. All the strains used reacted as expected, proving this new technique to be reliable in the identification of Brucella species. The method is less expensive and the...

The efficacy of monoclonal antibodies against T cell subsets in the therapy of experimental myocarditis caused by coxsackievirus B3 (CB3) was investigated. Two-week-old male C3H/He mice were inoculated with CB3 virus. Treatment was begun in the viremic stage (starting on the day of inoculation) in experiment 1 and in the later aviremic stage (starting on day 10) in experiment 2. Rat anti-mouse ...

We have had this study to evaluate the clinical profile of target-controlled infusion-based anesthesia using remifentanil and propofol. 116 ASA I-II patients undergoing elective laparoscopic cholecystectomy (LCH) were enrolled. TCI Remifentanil was set at 8 micrograms. L - 1 as target and TCI propofol at 4 mcg/ml throughout the whole procedure. The hemodynamics during induction of anesthesia an...

We have compared three bolus and infusion regimens of remifentanil on the cardiovascular response to laryngoscopy and orotracheal intubation in three groups of 20 ASA I-II female patients, in a randomized, double-blind study. Patients in group 1 received glycopyrolate 200 micrograms i.v. followed by a bolus dose of remifentanil 1 microgram kg-1 over 30 s and an infusion of remifentanil at a rat...

To define the iron absorptive phase across the intestinal mucosa of neonatal pigs, the final SgFe concentration ratio of serosal to mucosal medium of everted gut sac at 1 hr incubation was measured. With .2 btg carrier iron (ferric citrate with molecular weight of 1,500) per milliliter of both the serosal and mucosal medium, the duodenum transported the iron against the concentration gradient f...