Friable callus was initiated from shoot apices of Gloriosa superba L. on basal MS medium supplemented with 2, 4-D (4mg L(-1)) + Kn(5 mg L(-1)) + CH(10 mg L(-1)) + CW(20%). Subculture of callus on the same medium after 4-5 weeks showed induction of large number of somatic embryos, which was confirmed with histological studies. Development of embryoids in plantlet took place when the embryogenic ...

The experiment was conducted during May to December 2008 in the Biotechnology Laboratory of Bangladesh Agricultural University, Mymensingh to observe the callus induction, regeneration potentiality and to establish a suitable in vitro plantlet regeneration protocol of Corchorus olitorius. MS medium supplemented with different phytohormone concentrations and combinations were used to observe the...

The aim of this work was to develop protocols for micropropagation of selected Rubus cultivars – ‘Tulameen’ and ‘Black Jewel’ (Rubus idaeus L.), ‘Black Satin’ (Rubus fruticosus L.) and ‘Tayberry’ (Rubus fruticosus x Rubus idaeus). Nodal segments carrying dormant buds were used for in vitro culture establishment. For shoot initiation, MS medium containing 8 g*L phytoagar, 30 g*L sucrose, 1 mg*L ...

among pome fruit trees, quince is the most susceptible tree to fire blight. following selection of several promising quince cultivars, this research aimed at optimization of in vitro propagation protocol of the selected materials. therefore, several experiments were carried out for determination of the best culture medium and plant growth regulators for establishment, proliferation and root ind...

An efficient protocol for plant regeneration has been developed for crop improvement programs. The present study was designed to assess the regeneration response of cotyledonary node explant taken from the germinating seeds of peanut (Arachis hypogaea L.). Complete plants were regenerated from in vitro cultured sectioned cotyledonary nodes. Multiple shoots arose on 6-benzylaminopurine (BAP) sup...

The aim of this study was to develop methods for the in vitro propagation of Zantedeschia rehmannii. Zantedeschia rehmannii tuber fragments (1 cm2) containing eyes were soaked for 30 s in a solution containing 100 mg dm-3 L-ascorbic acid (AA) before transfer to culture vessels containing an MS medium supplemented with BAP (0 to 3 mg dm-3). Cultures were maintained in darkness. Soaking explants ...

A prerequisite for most plant transformation systems is an efficient and reliable method to regenerate phenotypically normal plants. The present studies were undertaken with a view to the effects of Benzyl amino purine (BAP) and Indol butyric acid (IBA) hormones and genotypes on in vitro germination of immature embryos in walnut (Juglans regia L.). The immature embryos of walnut were excised fr...