The structural and functional diversity of the human proteome is mediated by N- and O-linked glycosylations that define the individual properties of extracellular and membrane-associated proteins. In this study, we utilized different computational tools to perform in silico based genome-wide mapping of 1,117 human proteins and unravel the contribution of both penultimate and vicinal amino acids...

molecular modeling analysis of charge density and heat of fornation by pm3 method as well as c, h nmr and 2d-nmr measurements of histidine (substrate) and some of its derivatives as histidine decarboxylase inhibitors were performed. it was established that the atom, usually nitrogen, which forms internal aldimine with pyridoxal5 -phosphate (plp), (coenzyme), has negative and almost equal charge...

One of the most common techniques for separating mixtures of proteins is the two-dimensional polyacrylamide gel. In these gels, proteins are separated in one dimension based on their electromigration speeds through the gel (roughly determined by their molecular weights) and in another dimension by the pH at which their side chains cumulatively amount to a neutral charge, called the isoelectric ...

Small viral potassium (K+) channels represent with less than 100 amino acids (aa) per monomer the pore module of every K+ channel. The combination small size and robust function makes them ideal model systems to investigate basic structure/function correlations in channel pores. In prototype KcvPBCV-1 second TM helix ends C-terminus which last aa L94 plays an important role gating. A salt bridg...

one of the most dazzling events in the atmosphere is lightning. during updrafts in the life cycle of cumulus clouds, collision of graupels and ice crystals in the presence of liquid water results in vertical separation of electrical charges and lightning. there are four types of lightening depending on the location of discharge. the first type is cloud-to-ground lightning or fork lightning that...

The activation of Shaker K+ channels is steeply voltage dependent. To determine whether conserved charged amino acids in putative transmembrane segments S2, S3, and S4 contribute to the gating charge of the channel, the total gating charge movement per channel was measured in channels containing neutralization mutations. Of eight residues tested, four contributed significantly to the gating cha...

Native ferredoxin isolated from Clostridium acidi-urici cannot be acetylated and is not digested by carboxypeptidase A. However, apoferredoxin, free of iron and sulfide, can be acetplated and the COOH-terminal alanine and glutamine residues are quantitatively removed by carboxypeptidase A. Ferredoxin derivatives can be reconstituted from both modified proteins. The following derivatives were al...

The interaction between oppositely charged amino acids was studied by charge-transfer reversed-phase thin-layer chromatography. The dependence of the lipophilicity of Arg, Lys and Orn on the concentration of Glu, Asp, Gln and Asn in the eluent was considered to be related to the strength of interaction. The interaction of dibasic amino acids with Glu and Asp was stronger than with Gln and Asn. ...

The effect of various non-SH-containing amino acids on an electron spin resonance spectrum as well as on an optical absorption spectrum of an aqueous copper (II) solution was investigated. The data obtained indicate that a charge-transfer complex is formed with amino acids acting as electron donors. A plot of the wavelength of the CT-band vs. the pK values of the carboxyl group exhibits a strai...

In this study, a novel residue-residue contacts prediction approach based on evolutionary computation is presented. The prediction is based on four amino acids properties. In particular, we consider the hydrophobicity, the polarity, the charge and size of residues of amino acids. The prediction model consists of a set of rules that identifies contacts between amino acids. Results obtained confi...