The binding of five biologically important metals to serum proteins has been studied. After suitable radioactive isotopes were added to serum proteins separated and precipitated by two-dimensional immunoelectrophoresis, the sample plates were exposed to roentgenogram film. 59Fe bound to transferrin alone; 65Zn bound mostly to albumin, but also to another 12 proteins; 109Cd was mostly associated...

HeLa cells incubated in serum-free medium accumulated 59Fe ("non-transferrin iron") when incubated with either 59Fe-citrate, 59Fe-nitrilotriacetate, or 59Fe dissolved in Tricine ascorbate. Accumulation of iron was time-, concentration-, and Ca2+-dependent and was saturable. Uptake of non-transferrin (non-Tf) iron was transferrin-independent because of the fact that uptake occurred at pH 5.5, a ...

The objectives of the study were to determine the physiological and toxicological effects of chronic cadmium exposure on juvenile rainbow trout in soft water. Particular attention focused on acclimation, on comparison to an earlier hard water study, and on whether a gill surface binding model, originally developed in dilute soft water, could be applied in this water quality to fish chronically ...

Thyroid Benign Nodules (TBN) are the most common lesions of this endocrine gland. The etiology TBN is not clear. aim exploratory study was to examine differences in content bromine (Br), cooper (Cu), iron (Fe), iodine (I), rubidium (Rb), strontium (Sr), and zinc (Zn), as well I/Br, I/Cu, I/Fe, I/Rb, I/Sr, I/Zn ratios tissues normal thyroid TBN. tissue levels seven Trace Elements (TEs) were pros...

Previous logCd-based K XRF bone lead measurement systems have used an annular source of “‘Cd mounted coaxially with, and in front of, a 16 mm diameter hyperpure germanium detector. To take advantage of the advent of larger volume detectors, the IogCd fluorescing source was redesigned to allow its positioning at the center of the I detector face. The redesigned source is a cylinder of external d...

The backscatter fundamental parameter (BFP) method has been applied for determination of elements in loose powder samples utilizing double excitation with Fe and Cd radioisotope sources. The method has been used for laboratory determination of element concentrations in soil and geological samples. The combined excitation with Fe and Cd sources allows for improved detection limits of low-Z eleme...

A commercial-off-the-shelf (COTS) silicon carbide (4H-SiC) UV photodiode was electrically characterized and investigated as a low-cost spectroscopic photon counting detector of X-rays ?-rays. The coupled to custom-built low-noise charge-sensitive preamplifier, illuminated by 55Fe 109Cd radioisotope X-ray sources an 241Am ?-ray source, thus providing energies from 5.9 keV 59.5 keV. preamplifier ...