UNLABELLED CRISPRdirect is a simple and functional web server for selecting rational CRISPR/Cas targets from an input sequence. The CRISPR/Cas system is a promising technique for genome engineering which allows target-specific cleavage of genomic DNA guided by Cas9 nuclease in complex with a guide RNA (gRNA), that complementarily binds to a ∼ 20 nt targeted sequence. The target sequence require...

A diesel fuel spill was simulated on a 12-m(2) plot of agricultural land, at a concentration of 1 Lm(-2) of soil. The natural attenuation of Volatile Aromatic Hydrocarbons (VAHs) was monitored at different soil depths over a period of 50 days. The natural attenuation of VAHs in the surface layer would be attributed to two processes, namely: volatilisation (mainly linked to the boiling point of ...

The RNA-guided endonuclease Cas9 cleaves double-stranded DNA targets complementary to the guide RNA and has been applied to programmable genome editing. Cas9-mediated cleavage requires a protospacer adjacent motif (PAM) juxtaposed with the DNA target sequence, thus constricting the range of targetable sites. Here, we report the 1.7 Å resolution crystal structures of Cas9 from Francisella novici...

Streptococcus pyogenes Cas9 (SpCas9) is the most widely used genome editing tool in plants. The induced by SpCas9 strictly requires a canonical NGG protospacer-adjacent motif (PAM), significantly limiting its scope of application. Recently, five variants, SpCas9-NRRH, SpCas9-NRCH, SpCas9-NRTH, SpG, and SpRY, were developed to recognize non-canonical PAMs human cells. In this study, these varian...

Tandem repeats of certain trinucleotides show extensive intergenerational instability in humans that is associated with a class of genetic disorders known as the Triplet Expansion Diseases. This instability is thought to be a consequence of the formation of intrastrand structures, including hairpins, triplexes and tetraplexes, by the tandem repeats. I show here that CGG-repeats which are associ...

the aim of this study was to investigate the trends in mortality from road traffic accidents (rta) in fars province, southern iran.the age and sex-standardized mortality rate attributed to rta from 2004 to 2010 in fars province was calculated using world standard population. we also used linear regression and chi-squared tests.over the period of study (7 years), 12954 people died in rta. the ag...

P e ngg u n a an material yang kuat tetapi ringan sangat penting pada struktur pesawat terbang, salah satu digunakan terbang adalah komposit honeycomb sandwich panel. Tujuan dari penelitian ini untuk mengetahui karakteristik dengan membandingkan kekuatan tekan dan tekuk panel apabila core divariasikan ke bentuk berbeda asalnya, seperti terinspirasi alam beberapa peneliti sudah menggunakannya be...

This study aimed at comparing toxic element (Hg, Cd) bioaccumulation in relation to age for bottlenose (Tursiops truncatus) and striped dolphins (Stenella coeruleoalba) from Mediterranean and Atlantic waters. Metal concentrations were also measured in selected prey to infer metal exposure through the diet. As expected, Mediterranean prey exhibited the highest Hg levels, probably as a consequenc...