l-asparaginase is a candidate enzyme for anti-neoplastic agent againstacute lymphoblasticleukemia and also extensively use in the food industry for prevention of acrylamide formation. l-asparaginase is widely distributed among microorganisms. in this study, marine bacteria were isolated from persian gulf and screened for l-asparaginase activity. production of l-asparaginase was carried out by u...

bipolaris oryzae causes rice brown spot disease which reduces rice yield with substantial global impact. streptomycetes isolated from rice fields of guilan province, iran, showed antifungal activity against the tested pathogen. from five active streptomyces spp. isolates, isolate g showed high antagonistic activity. to determine its taxonomical identity, its colonies characterized morphological...

background :   vibrio species including v. cholera , v. mimicus , v. parahaemolyticus , and v. vulnificus may cause gastrointestinal diseases after seafood consumption, and wound infections in swimmers and fishermen after exposing to seawater. this study determined the prevalence of the four vibrio species in tonkabon and ramsar recreational beach water (approximately 200 meter far from the pla...

the identification of mycobacteria in the species level has great medical importance. biochemical tests are laborious and time-consuming, so new techniques could be used to identify the species. this research aimed to the comparison of biochemical and sequencing 16s rdna gene methods to identify nontuberculous mycobacteria in patients suspected to tuberculosis in golestan province which is the ...

background and purpose: cutaneous infections arise from a homogeneous group of keratinophilic fungi, known as dermatophytes. since these pathogenic dermatophytes are eukaryotes in nature, use of chemical antifungal agents for treatment may affect the host tissue cells. in this study, we aimed to evaluate the antifungal activity of actinomyces species against trichophyton mentagrophytes (abbrevi...

Using polymerase chain reaction (PCR), we examined 16S ribosomal DNA (rDNA) of Helicobacter species in liver tissue specimens obtained from 15 patients with hepatocellular carcinoma. Sixty percent (9 of 15) of these specimens were found to be positive for Helicobacter species. Four 16S rDNA fragments from positive PCR samples were directly sequenced. By sequence comparison, all were found to be...

BACKGROUND Cases of infective endocarditis (IE) require prompt etiological diagnosis for effective treatment. Molecular methods can aid in rapid and reliable diagnosis of culture-negative IE cases. We evaluated the utility of 16S rDNA PCR and sequencing in determining the causative agents of IE in valve tissues, especially when specimens were obtained after initiation of antimicrobial therapy. ...

Microbial translocation (MT) from the gut is implicated in driving immune activation, increasing morbidity and mortality in HIV. We used bacterial 16S rDNA PCR, Sanger sequencing, and high-throughput sequencing to identify microbial DNA in the bloodstream of HIV-infected children in London, United Kingdom. Blood samples were collected from sequential children attending the HIV clinic at Great O...

We have constructed a large fosmid library from a mesophilic anaerobic digester and explored its 16S rDNA diversity using a high-density filter DNA-DNA hybridization procedure. We identified a group of 16S rDNA sequences forming a new bacterial lineage named WWE3 (Waste Water of Evry 3). Only one sequence from the public databases shares a sequence identity above 80% with the WWE3 group which h...