introduction pterygium is a wing-shaped, vascular, fleshy growth that originates from the conjunctiva and can spread into the corneal limbus and beyond. beta irradiation after bare sclera surgery of primary pterygium is a simple, effective, and safe treatment, which reduces the risk of local recurrence. materials and methods dosimetric components of strontium-90 (90sr), phosphorous-32 (32p), an...

Exposure of 32P-labelled human platelets to ionophore A23187 results in an increased incorporation of 32P into polypeptides with apparent mol.wts. of 47000 (P47) and 20000 (P20), whereas exposure to prostaglandin El results in increased labelling of polypeptides with apparent mol.wts. of 24000 (P24) and 22000 (P22) [Haslam, Lynham & Fox (1979) Biochem. J. 178, 397-406]. Labelled platelets that ...

In order to characterize part of an active center of sodium and potassium ion transport adenosine triphosphatase, namely the active site of phosphorylation, the enzyme was phosphorylated with [32P]ATP and denatured with acid. The denatured [3SP]phosphoenzyme was digested to a limit [32P]phosphotripeptide with Pronase. The limit phosphotripeptide was compared with the synthetic model compounds, ...

In Escherichia coli, a two-component signal transduction system, consisting of the transmembrane sensor protein ArcB and its cognate cytoplasmic regulatory protein ArcA, controls the expression of genes encoding enzymes involved in aerobic respiration. ArcB belongs to a subclass of sensors that have not only a conserved histidine-containing transmitter domain but also a conserved aspartate-cont...

In vivo PTH administration to rats resulted in increased brain synaptosomal Ca++ transport, while parathyroidectomy (PTX) resulted in decreased transport. To determine the mechanism of action of PTH on Ca++ transport in rat brain synaptosomes, we performed transport studies by the Na-Ca exchanger and also measured cAMP generation in synaptosomes from PTX rats. Ca++ transport was studied after i...

Platelet-derived endothelial cell growth factor (PD-ECGF) is a 45-kDa peptide mitogen which is present in platelets and placenta and produced by certain cultured cell lines. Immunoprecipitation of A431 cells metabolically labeled with [32P]orthophosphate revealed the incorporation of 32P radioactivity into PD-ECGF. Phosphoamino acid analysis showed that serine residues of PD-ECGF were phosphory...

The uptake of [32P]phosphate by human, gel-filtered blood platelets and its incorporation into cytoplasmic ATP and polyphosphoinositides was studied. In unstimulated platelets, uptake was Na+o-dependent and saturable at approximately 20 nmol/min/10(11) cells with a half-maximal rate at 0.5 mM extracellular phosphate. Upon stimulation with thrombin or collagen, net influx of [32P]Pi was accelera...

Polyoma virus-transformed baby hamster kidney (pyBHK) cells were cultured in medium containing [32P]orthophosphate and 10% (vol/vol) fetal bovine serum. A 32P-labeled protein with an apparent molecular mass of 97 kDa was immunoprecipitated from cell lysates with antiserum to ADP-ribosylated elongation factor 2 (EF-2). The 32P labeling of the protein was enhanced by culturing cells in medium con...

Potato spindle tuber viroid (PSTV) was radioactively labeled during its synthesis in PSTV-infected tomato plants whose roots had been placed in nutrient solution containing [32P]orthophosphate. The 32P was incorporated into PSTV when the radioactive label was introduced into infected plants ‘2 weeks after inoculation, when systemic symptoms of PSTV started to appear. Synthesis of 32P-labeled PS...