Saquinavir (SQV) is a protease inhibitor widely used for the treatment of human immunodeficiency virus (HIV) infection. We profiled SQV metabolism in mice using a metabolomic approach. Thirty SQV metabolites were identified in mouse feces and urine, of which 20 are novel. Most metabolites observed in mice were recapitulated in human liver microsomes. Among these novel metabolites, one a-hydroxy...

The effect of rifampicin on the pharmacokinetics of fexofenadine enantiomers was examined in healthy subjects who received fexofenadine alone or with single or multiple doses of rifampicin (600 mg). A single coadministered dose of rifampicin significantly decreased the oral clearance (CLtot/F) and renal clearance (CLr) of Sand R-fexofenadine by 76 and 62%, and 73 and 62%, respectively. Even aft...

RNA interference (RNAi) is a powerful tool to study gene function in cultured cells. Transfected cell microarrays in principle allow high-throughput phenotypic analysis after gene knockdown by microscopy. But bottlenecks in imaging and data analysis have limited such high-content screens to endpoint assays in fixed cells and determination of global parameters such as viability. Here we have ove...

Toxoplasma gondii is an important pathogen in veterinary and human medicine. In this study, a new multiplex TaqMan real-time PCR for detection of T. gondii DNA was developed. This assay consisted of new sets of primers and probes which targeted B1 gene and ITS-1 region of T. gondii, with Vibrio cholera gene as internal control. The B1 gene primers were designed to detect T. gondii RH strain, wh...

Abstract Background Type 2 diabetes mellitus (DM) is associated with worsened clinical outcomes in hypertrophic cardiomyopathy (HCM) patients. The reasons for this adverse prognostic association are incompletely understood. Although distinct entities both HCM and DM share common features of impaired myocardial energetics coronary microvascular function. Purpose We sought to test the hypothesis ...

1 Université Pierre et Marie Curie-Paris 6, Centre de Recherche de l’Institut du Cerveau et de la Moelle épinière, UMR-S975; Inserm, U975, CNRS, UMR 7225, 75013 Paris, France 2 Centre national de référence des démences rares, 75013 Paris, France 3 Université Lyon I; Centre de Neurosciences Cognitives, CNRS UMR5229; Service de Neurologie C, Hôpital Neurologique Pierre Wertheimer, 69500 Bron, Fra...

In the past, the Baltic became seriously contaminated by organochlorines (1). The most dramatic increase occurred in the levels of the 2 major organochlorines, namely dichlorodiphenyltrichloroethane (DDT) and polychlorinated biphenyls (PCB) occurred after 1955 (2). A rapid decrease in concentrations of DDT compounds started during the beginning of the 1970s, whereas concentrations of PCB compou...

The aim of the study was to identify trends and dynamics changes in chemical biochemical properties soil induced by rotational grazing farmed deer. Soils pH content mineral nitrogen forms, total nitrogen, organic carbon were determined activity three enzymes. monitored soils slightly acidic neutral, with 1 mol KCldm−3 ranging from 6.37 7.03. contents carbon, range 9.42–19.37 gkg−1 1.13–2.24 gkg...

cation exchange capacity and specific surface area are among some of the important soil characteristics the direct measurement of which is laborious, costly and time consuming. therefore, fractal dimension of particle size distribution has been widely studied in relation with many such dynamic and static processes as transmission of water and solutes, water holding capacity, heat storage and co...