The vacuolar protein sorting (vps) system in the filamentous fungus Aspergillus oryzae, which has unique cell polarity and the ability to secrete large amounts of proteins, was evaluated by using mutants that missort vacuolar proteins into the medium. Vacuolar carboxypeptidase Y (CPY) fused with enhanced green fluorescent protein (EGFP) was used as a vacuolar marker. Twenty dfc (dim EGFP fluore...

Antigene oligonucleotides have the potential to regulate gene expression through site-specific DNA binding. However, in vivo applications have been hindered by inefficient cellular uptake, degradation, and strand displacement. Peptide nucleic acids (PNAs) address several of these problems, as they are resistant to degradation and bind DNA with high affinity. We designed two cationic pyrimidine ...

Clathrin heavy chain-deficient mutants (chcl) of Saccharomyces cerevisiae are viable but exhibit compromised growth rates. To investigate the role of clathrin in intercompartmental protein transport, two pathways have been monitored in chcl cells: transport of newly synthesized vacuolar proteins to the vacuole and receptor-mediated uptake of mating pheromone. Newly synthesized precursors of the...

A number of chemicals, including several organochlorine pesticides, have been identified as persistent organic pollutants (POPs). Here, the properties of chlorpyrifos (CPY; CAS No. 2921-88-2) and its active metabolite, chlorpyrifos oxon (CPYO; CAS No. 5598-15-2), are assessed relative to criteria for classification of compounds as persistent, bioaccumulative, and toxic substances (PBTs). The ma...

The use of a range of technical, symbolic and other means of representation for sensory evaluation contributes to high quality product development in the Food industry. Use of sensory evaluation techniques adapted from industrial practice would contribute to pupils' achievement of higher levels of attainment particularly in Attainment target 3: Planning & Making. Existing educational resources ...

Concentrations of CPY in surface waters are an integral determinant of risk to aquatic organisms. CPY has been measured in surface waters of the U.S. in several environmental monitoring programs and these data were evaluated to characterize concentrations, in relation to major areas of use and changes to the label since 2001, particularly the removal of domestic uses. Frequencies of detection a...

Lentil is a versatile and profitable pulse crop with high nutritional food feed values. The objectives of the study were to determine suitable locations for yield quality in terms production and/or breeding, identify promising genotypes. For this reason, five lentil genotypes evaluated multi-location network consisting ten diverse sites two consecutive growing seasons, seed (SY), other agronomi...

We have previously shown that the signal sequence of the Saccharomyces cerevisiae vacuolar protein carboxypeptidase Y (CPY) does not function in mammalian cells unless a glycine residue in the central core is replaced by leucine. Additional mutants were constructed to investigate the features of this hydrophobic core (h) region that are important for signal sequence function in mammalian cells....

Background The cytochrome P450 2C9 and 3A5 enzymes are predominantly found in the human liver, and have important functions in the metabolism of antiepileptics. Genetic polymorphisms in these genes are associated with altered enzymatic activities and may give rise to the risk of drug hypersensitivity. Therefore, we analyze the association between CBZ hypersensitivity reactions (CHR) and polymor...

Prior studies of the power spectra of GPS position time series have found pervasive seasonal signals against a power-law background of flicker noise plus white noise. Dong et al. (2002) estimated that less than half the observed GPS seasonal power can be explained by redistributions of geophysical fluid mass loads. Much of the residual variation is probably caused by unidentified GPS technique ...