background and objectives: meningoencephalitis in iranian children is frequent, but encephalitis is rare .the frequency of hhv-6 and hhv-7 in central nervous system diseases of our children is unclear. the aim of this study was searching the dna-s of hhv-6 & hhv-7 in csf samples of children with meningoencephalitis  materials and methods: a cross sectional study (2007-2009) was done in ped...

We looked for human herpesvirus 6 (HHV-6) and HHV-7 genomes in the cervixes of pregnant women in the late stages of their pregnancies. Of 72 samples collected with cervical swabs and amplified by nested PCR, we found that 14 (19.4%) and 2 (2.7%) contained detectable HHV-6 and HHV-7 genomes, respectively. The two samples in which HHV-7 DNA was detected also contained HHV-6 genomes. Hybridization...

AIM To investigate whether human herpesvirus 6 (HHV-6) can cause latent infection of liver tissue. METHODS Peripheral blood and liver tissue were collected from 25 living related liver transplant recipients at the time of transplantation. An avidin-biotin complex peroxidase method was used to identify HHV-6 antigen in the liver tissue. A nested polymerase chain reaction (PCR) was used to dete...

To determine the involvement of oxidative stress in the pathogenesis of acute encephalopathy associated with human herpesvirus-6 (HHV-6) infection, we measured the levels of oxidative stress markers 8-hydroxy-2'-deoxyguanosine (8-OHdG) and hexanoyl-lysine adduct (HEL), tau protein, and cytokines in cerebrospinal fluid (CSF) obtained from patients with HHV-6-associated acute encephalopathy (HHV-...

BACKGROUND Human herpesvirus-6 (HHV-6) has been associated with a diverse spectrum of central nervous system (CNS) diseases and reported glial tropism. OBJECTIVE To determine if HHV-6 is present in a series of pediatric brain tumors. STUDY DESIGN Pediatric gliomas from 88 untreated patients represented in a tissue microarray (TMA) were screened for HHV-6 by nested polymerase chain reaction ...

Immunoglobulin G to human herpesvirus 6 (HHV-6) and cytomegalovirus (CMV) in sera from solid organ recipients was measured by an enzyme-linked immunoassay (ELISA) before and after transplant. The HHV-6 ELISA was developed from glycine extracts of HHV-6-infected and uninfected HSB-2 cells. At a serum dilution of 1:500, 80 (91%) of 88 recipients were seropositive for HHV-6 before transplant, whil...

The objective was to evaluate the frequency of human herpesvirus 6 (HHV-6) DNA detection in the CSF of patients infected with HIV and its relation to brain disease and systemic HHV-6 infection. Nested polymerase chain reaction (PCR) was used to analyse CSF samples from 365 consecutive HIV infected patients with neurological symptoms. When available, plasma and brain tissues from patients whose ...

Few studies have examined the prevalence and cellular proclivity of latent human herpesvirus 6 (HHV-6) in healthy populations. Difficulties in detection of HHV-6 genome in different tissues using polymerase chain reaction (PCR) and immunohistochemistry (IHC) techniques have been reported by various researchers. We examined tonsils and adenoid tissues of 54 patients who had undergone tonsillecto...

In this study, we assessed the prevalence of human herpesvirus-7 (HHV-7) in 141 serum samples from children less than four years of age with exanthematic disease. All samples were negative for measles, rubella, dengue fever and parvovirus B19 infection. Testing for the presence of human herpesvirus-6 (HHV-6)-specific high avidity IgG antibodies by indirect immunofluorescence assay (IFA) reveale...