Chaperonins are essential for the folding of proteins in bacteria, mitochondria, and chloroplasts. We have functionally characterized the yeast mitochondrial chaperonins hsp60 and hsp10. In the presence of ADP, one molecule of hsp10 binds to hsp60 with an apparent Kd of 0.9 nM and a second molecule of hsp10 binds with a Kd of 24 nM. In the presence of ATP, the purified yeast chaperonins mediate...

Mortalin/mtHsp70 (mitochondrial Hsp70) and HSP60 (heat-shock protein 60) are heat-shock proteins that reside in multiple subcellular compartments, with mitochondria being the predominant one. In the present study, we demonstrate that the two proteins interact both in vivo and in vitro, and that the N-terminal region of mortalin is involved in these interactions. Suppression of HSP60 expression ...

The Ocean Drilling Program (ODP) has amassed large databases (including paleontological) as a result of its research on the geology of the ocean floor. ODP has begun to present its research results in electronic proceedings that will soon completely displace paper publications. Palaeontologia Electronica (PE) has pioneered the concept of an all-electronic paleontological journal with no paper e...

Forty Holstein dairy cows in their first and second lactation were used in a lactation study from 4-week prepartum through 14-week postpartum to investigate the potential effect of feeding different levels of ruminally protected methionine and choline on milk yield persistency of Holstein dairy cows. Cows were randomly assigned to receive one of the following treatments: 18 g/d of rumen-protect...

in this study, the effect of chemical modification of wood flour on the mechanical properties of polypropylene based composites was investigated using by dynamic mechanic thermal analysis. to meet this objective, the wood flour treated with chemical components including alkaline, acetic acid and benzoyl chloride, and then the chemically treated fibers were compounded with polypropylene at 180 °...

BACKGROUND With the introduction of an add-on handcycle, a crank system that can be placed in front of a wheelchair, handcycling was made widely available for daily life. With it, people go into town more easily, e.g. to do groceries; meet up with friends, etc. They have more independency and can be socially active. Our aim is to explore some settings of the handcycle, so that it can be optimal...

in this study canthaxanthin was produced by dietzia natronolimnaea hs1 and used for incorporation in niosomes after extraction. effect of rotational speed of rotary vacuum evaporator flask (100 and 150 rpm), canthaxanthin concentration (0, 1 and 2 microgram/ milliliter), concentration of poly ethylene glycol 400 (0, 0.1 and 0.2 %), surfactant type (tween 60 and 80, span 60 and 80) and surfactan...

This report contains details of over 100 Algol 60 test programs available from NPL which can be used to validate a compiler. Interpreting the results of the tests requires a good knowledge of Algol 60.