A dilute-acid pretreatment assisted with microwave irradiation was developed to efficiently fabricate nanocrystalline cellulose (NCC) the single use of cellulase from Aspergillus niger. The heating and diluted sulfuric acid solution (1, 2, 5, 10 wt. %) in considerably increased NCC yield subsequent enzymatic hydrolysis (feedstock enzyme ratio 2:1) a maximum value 84.4 wt.% less than without pre...

Dilute sulfuric acid hydrolysis was performed before the isolation of cellulose from Eulaliopsis binata. And then, the effects of dilute acid hydrolysis on composition and structure of the cellulose was studied in detail. The results indicated that hemicellulose was dissolved mostly and that the lignin-hemicellulose-cellulose interactions were also partially disrupted during the dilute acid hyd...

in this study, caffeic acid (ca) and its three derivatives including 3-caffeoylquinic acid (3-cqa, neochlorogenic acid), 4-caffeoylquinic acid (4-cqa, cryptochlorogenic acid), and 5-caffeoylquinic acid (5-cqa, chlorogenic acid) were identified in bupleurum chinense aerial parts using reverse-phase high-performance liquid chromatography (rp-hplc) with photodiode array (pda) detector, reference c...

194 CLINICAL CHEMISTRY, Vol. 25, No. 1, 1979 gas chromatography before and after acid (6 mol/L HC1) and alkaline (NaOH) hydrolysis. We frequently observe a peak in the lactic acid position after alkaline hydrolysis. After acid hydrolysis of the same urine no lactate isobserved, but a peak appears in the position of levulinic acid. The mass spectra of these two peaks were identical with those of...

Studies on the amino acid sequence of papain by means of the that hydrolysis of endopeptide linkages in dilute acid might peptides from the chymotryptic (l-3) and tryptic digests (4, 5) proceed predominantly by a mechanism, independent of hydrogen of the protein have yielded a considerable number of partial ions, involving un-ionized P-carboxyl groups of aspartyl residue. sequences. Four of the...

One hundred and twenty determinations of urinary estriol were carried out following acid hydrolysis and gas liquid chromatography. Through the use of a radioactive standard, it was shown that, with an increase in specific gravity (1.001 to 1.025), a steady linear decline of estriol recovery occurred (93% to 56%). The presence of glucose (1-5 gm./100 ml.) gradually diminished estriol recovery (7...

Yeast polygalacturonase catalyzes a random hydrolysis of pectic acid to a mixture of digalacturonic and galacturonic acids via a series of oligogalacturonides (1). This enzyme will be termed yeast endo-polygalacturonase to differentiate it from end group-hydrolyzing enzymes, which have been named exopolygalacturonases (2). Evidence has been presented that yeast endo-polygalacturonase is a singl...

enzymatic hydrolysis of yellowfin tuna visceral protein with neutrase has been carried out by response surface methodology using factorial design. peptide chain length was estimated as the response surface to the hydrolysis conditions (enzyme activity, reaction temperature, and reaction time). the tuna visceral protein hydrolysate had relatively high protein (74.56%) and low lipid content (1.86...

The administration of methenamine mandelate (Mandelamine) to pregnant women causes the levels of estriol in their urines to be unmeasurably low when acid hydrolysis is used. When enzyme hydrolysis replaces acid hydrolysis, normal estriol values are obtained. This result is attributed to the formaldehyde released by acid from hexamethylenetetramine, a constituent of the drug. The formaldehyde re...